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Bacteriophage Mu genome sequence: Analysis and comparison with Mu-like prophages in Haemophilus, Neisseria and Deinococcus

Morgan, GJ and Hatfull, GF and Casjens, S and Hendrix, RW (2002) Bacteriophage Mu genome sequence: Analysis and comparison with Mu-like prophages in Haemophilus, Neisseria and Deinococcus. Journal of Molecular Biology, 317 (3). 337 - 359. ISSN 0022-2836

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Abstract

We report the complete 36,717 bp genome sequence of bacteriophage Mu and provide an analysis of the sequence, both with regard to the new genes and other genetic features revealed by the sequence itself and by a comparison to eight complete or nearly complete Mu-like prophage genomes found in the genomes of a diverse group of bacteria. The comparative studies confirm that members of the Mu-related family of phage genomes are genetically mosaic with respect to each other, as seen in other groups of phages such as the phage λ-related group of phages of enteric hosts and the phage L5-related group of mycobacteriophages. Mu also possesses segments of similarity, typically gene-sized, to genomes of otherwise non-Mu-like phages. The comparisons show that some well-known features of the Mu genome, including the invertible segment encoding tail fiber sequences, are not present in most members of the Mu genome sequence family examined here, suggesting that their presence may be relatively volatile over evolutionary time. The head and tail-encoding structural genes of Mu have only very weak similarity to the corresponding genes of other well-studied phage types. However, these weak similarities, and in some cases biochemical data, can be used to establish tentative functional assignments for 12 of the head and tail genes. These assignments are strongly supported by the fact that the order of gene functions assigned in this way conforms to the strongly conserved order of head and tail genes established in a wide variety of other phages. We show that the Mu head assembly scaffolding protein is encoded by a gene nested in-frame within the C-terminal half of another gene that encodes the putative head maturation protease. This is reminiscent of the arrangement established for phage λ. © 2002 Elsevier Science Ltd.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Morgan, GJ
Hatfull, GFgfh@pitt.eduGFH
Casjens, S
Hendrix, RWrhx@pitt.eduRHX
Date: 1 January 2002
Date Type: Publication
Journal or Publication Title: Journal of Molecular Biology
Volume: 317
Number: 3
Page Range: 337 - 359
DOI or Unique Handle: 10.1006/jmbi.2002.5437
Schools and Programs: Dietrich School of Arts and Sciences > Biological Sciences
Refereed: Yes
ISSN: 0022-2836
MeSH Headings: Amino Acid Sequence; Bacteria--virology; Bacteriophage mu--genetics; Bacteriophage mu--isolation & purification; Base Sequence; Binding Sites; Consensus Sequence; DNA Gyrase--metabolism; Escherichia coli O157--virology; Evolution, Molecular; Genes, Viral--genetics; Genes, Viral--physiology; Genome, Viral; Haemophilus influenzae--virology; Mass Spectrometry; Neisseria meningitidis--virology; Physical Chromosome Mapping; Proviruses--genetics; Proviruses--isolation & purification; Transposases--metabolism; Viral Structural Proteins--genetics; Viral Structural Proteins--metabolism
PubMed ID: 11922669
Date Deposited: 19 Nov 2012 16:57
Last Modified: 02 Feb 2019 15:57
URI: http://d-scholarship.pitt.edu/id/eprint/16364

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