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Genomic characterization of mycobacteriophage giles: Evidence for phage acquisition of host DNA by illegitimate recombination

Morris, P and Marinelli, LJ and Jacobs-Sera, D and Hendrix, RW and Hatfull, GF (2008) Genomic characterization of mycobacteriophage giles: Evidence for phage acquisition of host DNA by illegitimate recombination. Journal of Bacteriology, 190 (6). 2172 - 2182. ISSN 0021-9193

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Abstract

A characteristic feature of bacteriophage genomes is that they are architecturally mosaic, with each individual genome representing a unique assemblage of individual exchangeable modules. Plausible mechanisms for generating mosaicism include homologous recombination at shared boundary sequences of module junctions, illegitimate recombination in a non-sequence-directed process, and site-specific recombination. Analysis of the novel mycobacteriophage Giles genome not only extends our current perspective on bacteriophage genetic diversity, with more than 60% of the genes unrelated to other mycobacteriophages, but offers novel insights into how mosaic genomes are created. In one example, the integration/excision cassette is atypically situated within the structural gene operon and could have moved there either by illegitimate recombination or more plausibly via integrase-mediated site-specific recombination. In a second example, a DNA segment has been recently acquired from the host bacterial chromosome by illegitimate recombination, providing further evidence that phage genomic mosaicism is generated by nontargeted recombination processes. Copyright © 2008, American Society for Microbiology. All Rights Reserved.


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Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Morris, P
Marinelli, LJ
Jacobs-Sera, Ddjs@pitt.eduDJS0000-0001-7838-3257
Hendrix, RWrhx@pitt.eduRHX
Hatfull, GFgfh@pitt.eduGFH
Date: 1 March 2008
Date Type: Publication
Journal or Publication Title: Journal of Bacteriology
Volume: 190
Number: 6
Page Range: 2172 - 2182
DOI or Unique Handle: 10.1128/jb.01657-07
Schools and Programs: Dietrich School of Arts and Sciences > Biological Sciences
Refereed: Yes
ISSN: 0021-9193
MeSH Headings: Base Sequence; DNA, Bacterial--genetics; Genome, Viral--genetics; Host-Pathogen Interactions; Microscopy, Electron; Molecular Sequence Data; Mycobacteriophages--genetics; Mycobacteriophages--physiology; Mycobacteriophages--ultrastructure; Mycobacterium smegmatis--genetics; Mycobacterium smegmatis--virology; Operon--genetics; Recombination, Genetic; Sequence Homology, Nucleic Acid
Other ID: NLM PMC2258872
PubMed Central ID: PMC2258872
PubMed ID: 18178732
Date Deposited: 03 Dec 2012 22:43
Last Modified: 02 Feb 2019 15:57
URI: http://d-scholarship.pitt.edu/id/eprint/16366

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