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GroEL1: A dedicated chaperone involved in mycolic acid biosynthesis during biofilm formation in mycobacteria

Ojha, A and Anand, M and Bhatt, A and Kremer, L and Jacobs, WR and Hatfull, GF (2005) GroEL1: A dedicated chaperone involved in mycolic acid biosynthesis during biofilm formation in mycobacteria. Cell, 123 (5). 861 - 873. ISSN 0092-8674

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Mycobacteria are unusual in encoding two GroEL paralogs, GroEL1 and GroEL2. GroEL2 is essential - presumably providing the housekeeping chaperone functions - while groEL1 is nonessential, contains the attB site for phage Bxb1 integration, and encodes a putative chaperone with unusual structural features. Inactivation of the Mycobacterium smegmatis groEL1 gene by phage Bxb1 integration allows normal planktonic growth but prevents the formation of mature biofilms. GroEL1 modulates synthesis of mycolates - long-chain fatty acid components of the mycobacterial cell wall - specifically during biofilm formation and physically associates with KasA, a key component of the type II Fatty Acid Synthase involved in mycolic acid synthesis. Biofilm formation is associated with elevated synthesis of short-chain (C56-C 68) fatty acids, and strains with altered mycolate profiles - including an InhA mutant resistant to the antituberculosis drug isoniazid and a strain overexpressing KasA - are defective in biofilm formation. ©2005 Elsevier Inc.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Ojha, Aano7@pitt.eduANO7
Anand, M
Bhatt, A
Kremer, L
Jacobs, WR
Hatfull, GFgfh@pitt.eduGFH
Date: 2 December 2005
Date Type: Publication
Journal or Publication Title: Cell
Volume: 123
Number: 5
Page Range: 861 - 873
DOI or Unique Handle: 10.1016/j.cell.2005.09.012
Schools and Programs: Dietrich School of Arts and Sciences > Biological Sciences
Refereed: Yes
ISSN: 0092-8674
MeSH Headings: 3-Oxoacyl-(Acyl-Carrier-Protein) Synthase--genetics; 3-Oxoacyl-(Acyl-Carrier-Protein) Synthase--metabolism; Amino Acid Sequence; Bacterial Proteins--genetics; Bacterial Proteins--metabolism; Biofilms; Chaperonin 60--genetics; Chaperonin 60--metabolism; Fatty Acids--chemistry; Fatty Acids--metabolism; Molecular Chaperones--genetics; Molecular Chaperones--metabolism; Molecular Sequence Data; Mycobacteriophages--genetics; Mycobacteriophages--metabolism; Mycobacterium smegmatis--genetics; Mycobacterium smegmatis--physiology; Mycolic Acids--chemistry; Mycolic Acids--metabolism; Sequence Alignment
PubMed ID: 16325580
Date Deposited: 19 Nov 2012 16:56
Last Modified: 22 Jun 2021 13:55


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