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Growth of Mycobacterium tuberculosis biofilms containing free mycolic acids and harbouring drug-tolerant bacteria.

Ojha, Anil K and Baughn, Anthony D and Sambandan, Dhinakaran and Hsu, Tsungda and Trivelli, Xavier and Guerardel, Yann and Alahari, Anuradha and Kremer, Laurent and Jacobs, William R and Hatfull, Graham F (2008) Growth of Mycobacterium tuberculosis biofilms containing free mycolic acids and harbouring drug-tolerant bacteria. Mol Microbiol, 69 (1). 164 - 174.

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Abstract

Successful treatment of human tuberculosis requires 6-9 months' therapy with multiple antibiotics. Incomplete clearance of tubercle bacilli frequently results in disease relapse, presumably as a result of reactivation of persistent drug-tolerant Mycobacterium tuberculosis cells, although the nature and location of these persisters are not known. In other pathogens, antibiotic tolerance is often associated with the formation of biofilms--organized communities of surface-attached cells--but physiologically and genetically defined M. tuberculosis biofilms have not been described. Here, we show that M. tuberculosis forms biofilms with specific environmental and genetic requirements distinct from those for planktonic growth, which contain an extracellular matrix rich in free mycolic acids, and harbour an important drug-tolerant population that persist despite exposure to high levels of antibiotics.

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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Ojha, Anil Kano7@pitt.eduANO7
Baughn, Anthony D
Sambandan, Dhinakaran
Hsu, Tsungda
Trivelli, Xavier
Guerardel, Yann
Alahari, Anuradha
Kremer, Laurent
Jacobs, William R
Hatfull, Graham Fgfh@pitt.eduGFH
Date: July 2008
Date Type: Publication
Journal or Publication Title: Mol Microbiol
Volume: 69
Number: 1
Page Range: 164 - 174
DOI or Unique Handle: 10.1111/j.1365-2958.2008.06274.x
Schools and Programs: Dietrich School of Arts and Sciences > Biological Sciences
Refereed: Yes
Uncontrolled Keywords: Antitubercular Agents, Biofilms, Carbon Dioxide, Drug Resistance, Bacterial, Humans, Iron, Lipids, Mycobacterium tuberculosis, Mycolic Acids, Plankton, Tuberculosis, Pulmonary, Zinc
Funders: NIAID NIH HHS (R01 AI064494), Wellcome Trust (066742)
MeSH Headings: Antitubercular Agents--pharmacology; Biofilms--drug effects; Biofilms--growth & development; Carbon Dioxide--metabolism; Drug Resistance, Bacterial; Humans; Iron--metabolism; Lipids--chemistry; Mycobacterium tuberculosis--drug effects; Mycobacterium tuberculosis--genetics; Mycobacterium tuberculosis--metabolism; Mycobacterium tuberculosis--physiology; Mycolic Acids--chemistry; Mycolic Acids--metabolism; Plankton--chemistry; Plankton--microbiology; Tuberculosis, Pulmonary--drug therapy; Tuberculosis, Pulmonary--microbiology; Zinc--metabolism
Other ID: NLM PMC2615189
PubMed Central ID: PMC2615189
PubMed ID: 18466296
Date Deposited: 05 Dec 2012 16:51
Last Modified: 31 Oct 2021 02:55
URI: http://d-scholarship.pitt.edu/id/eprint/16384

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