Ojha, Anil K and Baughn, Anthony D and Sambandan, Dhinakaran and Hsu, Tsungda and Trivelli, Xavier and Guerardel, Yann and Alahari, Anuradha and Kremer, Laurent and Jacobs, William R and Hatfull, Graham F
(2008)
Growth of Mycobacterium tuberculosis biofilms containing free mycolic acids and harbouring drug-tolerant bacteria.
Mol Microbiol, 69 (1).
164 - 174.
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Abstract
Successful treatment of human tuberculosis requires 6-9 months' therapy with multiple antibiotics. Incomplete clearance of tubercle bacilli frequently results in disease relapse, presumably as a result of reactivation of persistent drug-tolerant Mycobacterium tuberculosis cells, although the nature and location of these persisters are not known. In other pathogens, antibiotic tolerance is often associated with the formation of biofilms--organized communities of surface-attached cells--but physiologically and genetically defined M. tuberculosis biofilms have not been described. Here, we show that M. tuberculosis forms biofilms with specific environmental and genetic requirements distinct from those for planktonic growth, which contain an extracellular matrix rich in free mycolic acids, and harbour an important drug-tolerant population that persist despite exposure to high levels of antibiotics.
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Item Type: |
Article
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Status: |
Published |
Creators/Authors: |
Creators | Email | Pitt Username | ORCID  |
---|
Ojha, Anil K | ano7@pitt.edu | ANO7 | | Baughn, Anthony D | | | | Sambandan, Dhinakaran | | | | Hsu, Tsungda | | | | Trivelli, Xavier | | | | Guerardel, Yann | | | | Alahari, Anuradha | | | | Kremer, Laurent | | | | Jacobs, William R | | | | Hatfull, Graham F | gfh@pitt.edu | GFH | |
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Date: |
July 2008 |
Date Type: |
Publication |
Journal or Publication Title: |
Mol Microbiol |
Volume: |
69 |
Number: |
1 |
Page Range: |
164 - 174 |
DOI or Unique Handle: |
10.1111/j.1365-2958.2008.06274.x |
Schools and Programs: |
Dietrich School of Arts and Sciences > Biological Sciences |
Refereed: |
Yes |
Uncontrolled Keywords: |
Antitubercular Agents, Biofilms, Carbon Dioxide, Drug Resistance, Bacterial, Humans, Iron, Lipids, Mycobacterium tuberculosis, Mycolic Acids, Plankton, Tuberculosis, Pulmonary, Zinc |
Funders: |
NIAID NIH HHS (R01 AI064494), Wellcome Trust (066742) |
MeSH Headings: |
Antitubercular Agents--pharmacology; Biofilms--drug effects; Biofilms--growth & development; Carbon Dioxide--metabolism; Drug Resistance, Bacterial; Humans; Iron--metabolism; Lipids--chemistry; Mycobacterium tuberculosis--drug effects; Mycobacterium tuberculosis--genetics; Mycobacterium tuberculosis--metabolism; Mycobacterium tuberculosis--physiology; Mycolic Acids--chemistry; Mycolic Acids--metabolism; Plankton--chemistry; Plankton--microbiology; Tuberculosis, Pulmonary--drug therapy; Tuberculosis, Pulmonary--microbiology; Zinc--metabolism |
Other ID: |
NLM PMC2615189 |
PubMed Central ID: |
PMC2615189 |
PubMed ID: |
18466296 |
Date Deposited: |
05 Dec 2012 16:51 |
Last Modified: |
31 Oct 2021 02:55 |
URI: |
http://d-scholarship.pitt.edu/id/eprint/16384 |
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