Peña, CEA and Kahlenberg, JM and Hatfull, GF
(2000)
Assembly and activation of site-specific recombination complexes.
Proceedings of the National Academy of Sciences of the United States of America, 97 (14).
7760 - 7765.
ISSN 0027-8424
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Abstract
Site-specific recombination is responsible for a broad range of biological phenomena, including DNA inversion, resolution of transposition intermediates, and the integration and excision of bacteriophage genomes. Integration of mycobacteriophage L5 is catalyzed by a phage-encoded integrase with recombination occurring between specific attachment sites on the phage and mycobacterial chromosomes (attP and attB, respectively). Although some site-specific recombination systems simply involve binding of the recombinase to the sites of strand exchange, synapsis, and recombination, phage systems typically require the assembly of higher-order structures within which the recombinational potential of integrase is activated. The requirement for these structures derives from the necessity to regulate the directionality of recombination - either integration or excision - which must be closely coordinated with other aspects of the phage growth cycles. We show herein that there are multiple pathways available for the assembly of L5 recombination complexes, including the early synapsis of the attP and attB DNAs. This process is in contrast to the model for lambda integration and illustrates the different usage of molecular machineries to accomplish the same biological outcome.
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Details
Item Type: |
Article
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Status: |
Published |
Creators/Authors: |
Creators | Email | Pitt Username | ORCID  |
---|
Peña, CEA | | | | Kahlenberg, JM | | | | Hatfull, GF | gfh@pitt.edu | GFH | |
|
Date: |
5 July 2000 |
Date Type: |
Publication |
Journal or Publication Title: |
Proceedings of the National Academy of Sciences of the United States of America |
Volume: |
97 |
Number: |
14 |
Page Range: |
7760 - 7765 |
DOI or Unique Handle: |
10.1073/pnas.140014297 |
Schools and Programs: |
Dietrich School of Arts and Sciences > Biological Sciences |
Refereed: |
Yes |
ISSN: |
0027-8424 |
MeSH Headings: |
DNA, Bacterial--metabolism; DNA, Viral--metabolism; Integrases--metabolism; Models, Genetic; Mycobacterium--virology; Recombination, Genetic |
Other ID: |
NLM PMC16618 |
PubMed Central ID: |
PMC16618 |
PubMed ID: |
10869430 |
Date Deposited: |
05 Dec 2012 16:41 |
Last Modified: |
02 Feb 2019 15:57 |
URI: |
http://d-scholarship.pitt.edu/id/eprint/16485 |
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