A peptidoglycan hydrolase motif within the mycobacteriophage TM4 tape measure protein promotes efficient infection of stationary phase cells

Piuri, M and Hatfull, GF (2006) A peptidoglycan hydrolase motif within the mycobacteriophage TM4 tape measure protein promotes efficient infection of stationary phase cells. Molecular Microbiology, 62 (6). 1569 - 1585. ISSN 0950-382X

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Abstract

The predominant morphotype of mycobacteriophage virions has a DNA-containing capsid attached to a long flexible non-contractile tail, features characteristic of the Siphoviridae. Within these phage genomes the tape measure protein (tmp) gene can be readily identified due to the well-established relationship between the length of the gene and the length of the phage tail - because these phages typically have long tails, the tmp gene is usually the largest gene in the genome. Many of these mycobacteriophage Tmp's contain small motifs with sequence similarity to host proteins. One of these motifs (motif 1) corresponds to the Rpf proteins that have lysozyme activity and function to stimulate growth of dormant bacteria, while the others (motifs 2 and 3) are related to proteins of unknown function, although some of the related proteins of the host are predicted to be involved in cell wall catabolism. We show here that motif 3-containing proteins have peptidoglycan-hydrolysing activity and that while this activity is not required for phage viability, it facilitates efficient infection and DNA injection into stationary phase cells. Tmp's of mycobacteriophages may thus have acquired these motifs in order to avoid a selective disadvantage that results from changes in peptidoglycan in non-growing cells. © 2006 The Authors.

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Details

Item Type:	Article
Status:	Published
Creators/Authors:	Creators Email Pitt Username ORCID Piuri, M Hatfull, GF gfh@pitt.edu GFH
Date:	1 December 2006
Date Type:	Publication
Journal or Publication Title:	Molecular Microbiology
Volume:	62
Number:	6
Page Range:	1569 - 1585
DOI or Unique Handle:	10.1111/j.1365-2958.2006.05473.x
Schools and Programs:	Dietrich School of Arts and Sciences > Biological Sciences
Refereed:	Yes
ISSN:	0950-382X
MeSH Headings:	Amino Acid Motifs; Amino Acid Sequence; Blotting, Western; Electrophoresis, Polyacrylamide Gel; Genome, Viral--genetics; Luciferases--genetics; Luciferases--metabolism; Microscopy, Electron; Models, Biological; Molecular Sequence Data; Mycobacteriophages--genetics; Mycobacteriophages--growth & development; Mycobacteriophages--ultrastructure; Mycobacterium smegmatis--drug effects; Mycobacterium smegmatis--metabolism; Mycobacterium smegmatis--virology; N-Acetylmuramoyl-L-alanine Amidase--genetics; N-Acetylmuramoyl-L-alanine Amidase--metabolism; Protein Structure, Tertiary; Recombinant Fusion Proteins--genetics; Recombinant Fusion Proteins--metabolism; Sequence Homology, Amino Acid; Vancomycin--pharmacology; Viral Proteins--genetics; Viral Proteins--metabolism
Other ID:	NLM PMC1796659
PubMed Central ID:	PMC1796659
PubMed ID:	17083467
Date Deposited:	12 Dec 2012 20:22
Last Modified:	02 Feb 2019 15:57
URI:	http://d-scholarship.pitt.edu/id/eprint/16663

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