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Evaluation of fluoromycobacteriophages for detecting drug resistance in Mycobacterium tuberculosis

Rondón, L and Piuri, M and Jacobs, WR and De Waard, J and Hatfull, GF and Takiff, HE (2011) Evaluation of fluoromycobacteriophages for detecting drug resistance in Mycobacterium tuberculosis. Journal of Clinical Microbiology, 49 (5). 1838 - 1842. ISSN 0095-1137

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We tested a new method for detecting drug-resistant strains of Mycobacterium tuberculosis that uses a TM4 mycobacteriophage phAE87::hsp60-EGFP (EGFP-phage) engineered to contain the gene encoding enhanced green fluorescent protein (EGFP). After promising results in preliminary studies, the EGFP-phage was used to detect isoniazid (INH), rifampin (RIF), and streptomycin (STR) resistance in 155 strains of M. tuberculosis, and the results were compared to the resazurin microplate technique, with the proportion method serving as the reference standard. The resazurin technique yielded sensitivities of 94% for INH and RIF and 98% for STR and specificities of 97% for INH, 95% for RIF, and 98% for STR. The sensitivity of EGFP-phage was 94% for all three antibiotics, with specificities of 90% for INH, 93% for RIF, and 95% for STR. The EGFP-phage results were available in 2 days for RIF and STR and in 3 days for INH, with an estimated cost of ∼2$ to test the three antibiotics. Using a more stringent criterion for resistance improved the specificity of the EGFP-phage for INH and RIF without affecting the sensitivity. In preliminary studies, the EGFP-phage could also effectively detect resistance to the fluoroquinolones. The EGFP-phage method has the potential to be a valuable rapid and economic screen for detecting drug-resistant tuberculosis if the procedure can be simplified, if it can be adapted to clinical material, and if its sensitivity can be improved. Copyright © 2011, American Society for Microbiology. All Rights Reserved.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Rondón, L
Piuri, M
Jacobs, WR
De Waard, J
Hatfull, GFgfh@pitt.eduGFH
Takiff, HE
Date: 1 May 2011
Date Type: Publication
Journal or Publication Title: Journal of Clinical Microbiology
Volume: 49
Number: 5
Page Range: 1838 - 1842
DOI or Unique Handle: 10.1128/jcm.02476-10
Schools and Programs: Dietrich School of Arts and Sciences > Biological Sciences
Refereed: Yes
ISSN: 0095-1137
MeSH Headings: Antitubercular Agents--pharmacology; Drug Resistance, Bacterial; Fluorometry; Green Fluorescent Proteins--genetics; Green Fluorescent Proteins--metabolism; Humans; Microbial Sensitivity Tests--methods; Microbial Viability--drug effects; Mycobacteriophages--genetics; Mycobacteriophages--growth & development; Mycobacterium tuberculosis--drug effects; Mycobacterium tuberculosis--isolation & purification; Mycobacterium tuberculosis--virology; Sensitivity and Specificity; Staining and Labeling--methods; Tuberculosis--microbiology
Other ID: NLM PMC3122682
PubMed Central ID: PMC3122682
PubMed ID: 21346042
Date Deposited: 12 Dec 2012 20:15
Last Modified: 28 Sep 2022 13:28


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