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Mycobacteriophages BPs, angel and halo: Comparative genomics reveals a novel class of ultra-small mobile genetic elements

Sampson, T and Broussard, GW and Marinelli, LJ and Jacobs-Sera, D and Ray, M and Ko, CC and Russell, D and Hendrix, RW and Hatfull, GF (2009) Mycobacteriophages BPs, angel and halo: Comparative genomics reveals a novel class of ultra-small mobile genetic elements. Microbiology, 155 (9). 2962 - 2977. ISSN 1350-0872

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Mycobacteriophages BPs, Angel and Halo are closely related viruses isolated from Mycobacterium smegmatis, and possess the smallest known mycobacteriophage genomes, 41 901 bp, 42 289 bp and 41 441 bp, respectively. Comparative genome analysis reveals a novel class of ultra-small mobile genetic elements; BPs and Halo each contain an insertion of the proposed mobile elements MPME1 and MPME2, respectively, at different locations, while Angel contains neither. The close similarity of the genomes provides a comparison of the pre- and post-integration sequences, revealing an unusual 6 bp insertion at one end of the element and no target duplication. Nine additional copies of these mobile elements are identified in a variety of different contexts in other mycobacteriophage genomes. In addition, BPs, Angel and Halo have an unusual lysogeny module in which the repressor and integrase genes are closely linked. The attP site is located within the repressor-coding region, such that prophage formation results in expression of a C-terminally truncated, but active, form of the repressor. © 2009 SGM.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Sampson, T
Broussard, GW
Marinelli, LJ
Jacobs-Sera, Ddjs@pitt.eduDJS0000-0001-7838-3257
Ray, M
Ko, CC
Russell, Ddar78@pitt.eduDAR780000-0001-6894-2748
Hendrix, RWrhx@pitt.eduRHX
Hatfull, GFgfh@pitt.eduGFH
Date: 14 September 2009
Date Type: Publication
Journal or Publication Title: Microbiology
Volume: 155
Number: 9
Page Range: 2962 - 2977
DOI or Unique Handle: 10.1099/mic.0.030486-0
Schools and Programs: Dietrich School of Arts and Sciences > Biological Sciences
Refereed: Yes
ISSN: 1350-0872
MeSH Headings: Attachment Sites, Microbiological; Base Sequence; DNA, Viral--analysis; DNA, Viral--genetics; Genes, Viral; Genetic Variation; Genomics; Interspersed Repetitive Sequences; Lysogeny--genetics; Microscopy, Electron, Transmission; Molecular Sequence Data; Mycobacteriophages--genetics; Mycobacteriophages--isolation & purification; Mycobacteriophages--ultrastructure; Mycobacterium smegmatis--virology; Mycobacterium tuberculosis--virology; Sequence Analysis, DNA
Other ID: NLM NIHMS176946, NLM PMC2833263
PubMed Central ID: PMC2833263
PubMed ID: 19556295
Date Deposited: 12 Dec 2012 20:14
Last Modified: 02 Feb 2019 15:57


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