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CYSTEINE AND SPIN LABEL MODIFICATION AS A MEANS TO EXPLORE PROTEIN DYNAMICS AND STRUCTURE

McGoff, Marshall (2013) CYSTEINE AND SPIN LABEL MODIFICATION AS A MEANS TO EXPLORE PROTEIN DYNAMICS AND STRUCTURE. Master's Thesis, University of Pittsburgh. (Unpublished)

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Abstract

The goal of this work is to expand upon the current labeling technology available in the field of site directed spin labeling (SDSL) by cysteine and spin label modification. Multiple mutants of the B1 immunoglobulin-binding domain of protein G (GB1) were expressed, purified, characterized, and described herein. The synthesis and full characterization is described for several initial compounds along the pathway to a more rigid linker with less orientational freedom. The modification of cysteine using O-mesitylenesulfonylhydroxylamine (MSH) to form dehydroalanine with subsequent attachment of a thiol is detailed in this work. Electron Spin Resonance (ESR) spectra were collected as a means for comparison to newly designed labels. Future directions for the project include the completion of the synthesis and incorporation of new spin labels using dehydroalanine.

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Details

Item Type: University of Pittsburgh ETD
Status: Unpublished
Creators/Authors:
CreatorsEmailPitt UsernameORCID
McGoff, Marshallmsm73@pitt.eduMSM73
ETD Committee:
TitleMemberEmail AddressPitt UsernameORCID
Committee ChairSaxena, Sunilsksaxena@pitt.eduSKSAXENA
Committee MemberHorne, W. Sethhorne@pitt.eduHORNE
Committee MemberGarrett-Roe, Seansgr@pitt.eduSGR
Date: 25 January 2013
Date Type: Publication
Defense Date: 19 November 2012
Approval Date: 25 January 2013
Submission Date: 3 December 2012
Access Restriction: 5 year -- Restrict access to University of Pittsburgh for a period of 5 years.
Number of Pages: 70
Institution: University of Pittsburgh
Schools and Programs: Dietrich School of Arts and Sciences > Chemistry
Degree: MS - Master of Science
Thesis Type: Master's Thesis
Refereed: Yes
Uncontrolled Keywords: ESR, MSH, GB1
Date Deposited: 25 Jan 2013 14:18
Last Modified: 25 Jan 2018 06:15
URI: http://d-scholarship.pitt.edu/id/eprint/16737

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