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Genomic and structural analysis of Syn9, a cyanophage infecting marine Prochlorococcus and Synechococcus

Weigele, PR and Pope, WH and Pedulla, ML and Houtz, JM and Smith, AL and Conway, JF and King, J and Hatfull, GF and Lawrence, JG and Hendrix, RW (2007) Genomic and structural analysis of Syn9, a cyanophage infecting marine Prochlorococcus and Synechococcus. Environmental Microbiology, 9 (7). 1675 - 1695. ISSN 1462-2912

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Abstract

Cyanobacteriophage Syn9 is a large, contractile-tailed bacteriophage infecting the widespread, numerically dominant marine cyanobacteria of the genera Prochlorococcus and Synechococcus. Its 177 300 bp genome sequence encodes 226 putative proteins and six tRNAs. Experimental and computational analyses identified genes likely involved in virion formation, nucleotide synthesis, and DNA replication and repair. Syn9 shows significant mosaicism when compared with related cyanophages S-PM2, P-SSM2 and P-SSM4, although shared genes show strong purifying selection and evidence for large population sizes relative to other phages. Related to coliphage T4 - which shares 19% of Syn9's genes - Syn9 shows evidence for different patterns of DNA replication and uses homologous proteins to assemble capsids with a different overall structure that shares topology with phage SPO1 and herpes virus. Noteworthy bacteria-related sequences in the Syn9 genome potentially encode subunits of the photosynthetic reaction centre, electron transport proteins, three pentose pathway enzymes and two tryptophan halogenases. These genes suggest that Syn9 is well adapted to the physiology of its photosynthetic hosts and may affect the evolution of these sequences within marine cyanobacteria. © 2007 The Authors.

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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Weigele, PR
Pope, WHwelkin@pitt.eduWELKIN0000-0002-2012-2924
Pedulla, ML
Houtz, JM
Smith, AL
Conway, JFJames.Conway@pitt.eduJXC100
King, J
Hatfull, GFgfh@pitt.eduGFH
Lawrence, JGjlawrenc@pitt.eduJLAWRENC
Hendrix, RWrhx@pitt.eduRHX
Date: 1 July 2007
Date Type: Publication
Journal or Publication Title: Environmental Microbiology
Volume: 9
Number: 7
Page Range: 1675 - 1695
DOI or Unique Handle: 10.1111/j.1462-2920.2007.01285.x
Schools and Programs: Dietrich School of Arts and Sciences > Biological Sciences
Refereed: Yes
ISSN: 1462-2912
MeSH Headings: Bacteriophages--genetics; Bacteriophages--ultrastructure; Base Sequence; DNA Replication--genetics; Electrophoresis, Polyacrylamide Gel; Energy Metabolism--genetics; Evolution, Molecular; Gene Expression Regulation, Viral--genetics; Genes, Viral--genetics; Genome, Viral--genetics; Mass Spectrometry; Microscopy, Electron; Molecular Sequence Data; Prochlorococcus--virology; Sequence Analysis, DNA; Species Specificity; Synechococcus--virology; Virion--genetics
PubMed ID: 17564603
Date Deposited: 14 Jan 2013 16:13
Last Modified: 02 Feb 2019 16:58
URI: http://d-scholarship.pitt.edu/id/eprint/16740

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