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Photonic crystal optrode sensor for detection of Pb<sup>2+</sup> in high ionic strength environments

Reese, CE and Asher, SA (2003) Photonic crystal optrode sensor for detection of Pb<sup>2+</sup> in high ionic strength environments. Analytical Chemistry, 75 (15). 3915 - 3918. ISSN 0003-2700

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We developed an optrode sensing device that utilizes a polymerized colloidal array (PCCA) photonic crystal material. This array diffracts light in the visible spectral region due to the periodic spacing of colloidal particles. The PCCA changes diffraction wavelength due to binding of Pb2+ to an 18-crown-6 ether molecular recognition agent. This optrode consists of a probe assembly that contains the PCCA Pb2+ sensing film. An inexpensive, commercial diode array spectrometer and a fiber-optic reflectance probe monitors the wavelength of light back diffracted by the PCCA. Liquid inlet and outlet connections are provided to introduce the sample solution and to exchange out nonbinding ions. In low ionic strength solutions, diffraction wavelength shifts are actuated by the binding of the Pb2+ to the crown ether to immobilize the Pb2+ counterions. In these low ionic strength solutions, a Donnan potential forms to cause an osmotic pressure, which swells the PCCA in proportion to the number density of bound Pb2+. This Donnan potential disappears at high ionic strengths. Thus, no response of the PCCA occurs. Our optrode design allows for the fast removal of nonbound ions from the PCCA by washing with pure water. Since the bound Pb2+ ions have a slow off rate from the crown ether, the bound Pb2+ PCCA diffraction transiently red shifts during washing, directly in proportion to the sample Pb2+ concentration. This transient diffraction red-shift can be used to quantitatively determine Pb2+ concentrations in high ionic strength solutions such as bodily fluids.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Reese, CE
Asher, SAasher@pitt.eduASHER
Date: 1 August 2003
Date Type: Publication
Journal or Publication Title: Analytical Chemistry
Volume: 75
Number: 15
Page Range: 3915 - 3918
DOI or Unique Handle: 10.1021/ac034276b
Schools and Programs: Dietrich School of Arts and Sciences > Chemistry
Refereed: Yes
ISSN: 0003-2700
MeSH Headings: Chemistry Techniques, Analytical--instrumentation; Chemistry Techniques, Analytical--methods; Colloids; Hydrogels; Lead--analysis; Osmolar Concentration; Polymers; Sodium Chloride--chemistry; Spectrophotometry; Time Factors
PubMed ID: 14572062
Date Deposited: 23 Jan 2013 21:45
Last Modified: 22 Jun 2021 13:55


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