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Chikungunya Virus Glycoproteins Mediate Viral Entry and Cellular Fusion

Rinchuse, Derek (2013) Chikungunya Virus Glycoproteins Mediate Viral Entry and Cellular Fusion. Master's Thesis, University of Pittsburgh. (Unpublished)

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Abstract

The Chikungunya virus (CHIKV) has currently been identified in over 40 countries and in 2008 was listed as a US National Institute of Allergy and Infectious Diseases (NIAID) category C priority pathogen. Outbreaks of the virus have been documented as early as 1779 and frequent outbreaks have been reported through 1960-2003, most notably in Reunion Island, a French overseas department in the Indian Ocean. Out of a total population of 785,000 in Reunion Island, 300,000 cases were reported including a total of 237 deaths. Numerous aspects of the viral life cycle are unknown, with no current vaccine the implementation of more research and dissemination of more knowledge is of great public health importance. A CHIKV construct was synthesized by Genewiz, containing CHIKV structural proteins in pcDNA 3.1. This construct was used to create CHIKV pseudo-viral particles with a luciferase based reporter. The pseudo-virus was used to survey many cell lines for permissivity to infection. This construct was also used to create 3 other constructs containing CHIKV E1, E2, and E3 individually. These constructs were used individually and in combination with each other to create pseudo-viruses for cellular infection. The cell-cell fusion capabilities of the full CHIKV construct along with the individual envelope proteins were also tested in a Cre-Lox system.


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Details

Item Type: University of Pittsburgh ETD
Status: Unpublished
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Rinchuse, Derekdlrinchuse@gmail.com
ETD Committee:
TitleMemberEmail AddressPitt UsernameORCID
Thesis AdvisorWang, Tianyitywang@pitt.eduTYWANG
Committee MemberHartman, Amyhartman2@pitt.eduHARTMAN2
Committee MemberKlimstra, Williamklimstra@pitt.eduKLIMSTRA
Date: 29 January 2013
Date Type: Publication
Defense Date: 31 October 2012
Approval Date: 29 January 2013
Submission Date: 24 January 2013
Access Restriction: 5 year -- Restrict access to University of Pittsburgh for a period of 5 years.
Number of Pages: 64
Institution: University of Pittsburgh
Schools and Programs: Graduate School of Public Health > Infectious Diseases and Microbiology
Degree: MS - Master of Science
Thesis Type: Master's Thesis
Refereed: Yes
Uncontrolled Keywords: Chikungunya Virus
Date Deposited: 29 Jan 2013 22:16
Last Modified: 01 Jan 2018 06:15
URI: http://d-scholarship.pitt.edu/id/eprint/17191

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