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Acetylcholinesterase-based organophosphate nerve agent sensing photonic crystal

Walker, JP and Asher, SA (2005) Acetylcholinesterase-based organophosphate nerve agent sensing photonic crystal. Analytical Chemistry, 77 (6). 1596 - 1600. ISSN 0003-2700

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We developed a polymerized crystalline colloidal array (PCCA) photonic crystal sensing material that senses the organophosphorus compound parathion at ultratrace concentrations in aqueous solutions. A periodic array of colloidal particles is embedded in a hydrogel network with a lattice spacing such that it Bragg diffracts visible light. The molecular recognition agent for the sensor is the enzyme acetylcholinesterase (AChE), which binds organophosphorus compounds irreversibly, creating an anionic phosphonyl species. This charged species creates a Donnan potential, which swells the hydrogel network, which increases the embedded particle array lattice spacing and causes a red-shift in the wavelength of light diffracted. The magnitude of the diffraction red-shift is proportional to the amount of bound parathion. These AChE-PCCAs act as dosimeters for parathion since it irreversibly binds. Parathion concentrations as low as 4.26 fM are easily detected. © 2005 American Chemical Society.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Walker, JP
Asher, SAasher@pitt.eduASHER
Date: 15 March 2005
Date Type: Publication
Journal or Publication Title: Analytical Chemistry
Volume: 77
Number: 6
Page Range: 1596 - 1600
DOI or Unique Handle: 10.1021/ac048562e
Schools and Programs: Dietrich School of Arts and Sciences > Chemistry
Refereed: Yes
ISSN: 0003-2700
MeSH Headings: Acetylcholinesterase--metabolism; Biosensing Techniques--methods; Chemical Warfare Agents--analysis; Colloids; Crystallization; Parathion--analysis; Polymers
PubMed ID: 15762562
Date Deposited: 08 Feb 2013 21:15
Last Modified: 02 Feb 2019 16:55


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