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UV resonance raman finds peptide bond-Arg side chain electronic interactions

Sharma, B and Asher, SA (2011) UV resonance raman finds peptide bond-Arg side chain electronic interactions. Journal of Physical Chemistry B, 115 (18). 5659 - 5664. ISSN 1520-6106

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We measured the UV resonance Raman excitation profiles and Raman depolarization ratios of the arginine (Arg) vibrations of the amino acid monomer as well as Arg in the 21-residue predominantly alanine peptide AAAAA(AAARA)3A (AP) between 194 and 218 nm. Excitation within the π → π* peptide bond electronic transitions result in UVRR spectra dominated by amide peptide bond vibrations. The Raman cross sections and excitation profiles indicate that the Arg side chain electronic transitions mix with the AP peptide bond electronic transitions. The Arg Raman bands in AP exhibit Raman excitation profiles similar to those of the amide bands in AP which are conformation specific. These Arg excitation profiles distinctly differ from the Arg monomer. The Raman depolarization ratios ofArg in monomeric solution are quite simple with p = 0.33 indicating enhancement by a single electronic transition. In contrast, we see very complex depolarization ratios of Arg in AP that indicate that the Arg residues are resonance enhanced by multiple electronic transitions. © 2011 American Chemical Society.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Sharma, B
Asher, SAasher@pitt.eduASHER
Date: 12 May 2011
Date Type: Publication
Journal or Publication Title: Journal of Physical Chemistry B
Volume: 115
Number: 18
Page Range: 5659 - 5664
DOI or Unique Handle: 10.1021/jp112174s
Schools and Programs: Dietrich School of Arts and Sciences > Chemistry
Refereed: Yes
ISSN: 1520-6106
MeSH Headings: Amino Acid Sequence; Arginine--chemistry; Electrons; Molecular Sequence Data; Peptides--chemistry; Quantum Theory; Solutions--chemistry; Spectrum Analysis, Raman
Other ID: NLM NIHMS281091, NLM PMC3088768
PubMed Central ID: PMC3088768
PubMed ID: 21410150
Date Deposited: 08 Feb 2013 20:54
Last Modified: 22 Jun 2021 13:55


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