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Progress in developing polymerized crystalline colloidal array sensors for point-of-care detection of myocardial ischemia

Baca, JT and Finegold, DN and Asher, SA (2008) Progress in developing polymerized crystalline colloidal array sensors for point-of-care detection of myocardial ischemia. Analyst, 133 (3). 385 - 390. ISSN 0003-2654

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The difficulty of rapid, definitive diagnosis of myocardial ischemia leads to unnecessary hospital admissions and treatment delays. Previously, decreased metal binding affinity in human serum was investigated as a marker for myocardial ischemia. Polymerized Crystalline Colloidal Array (PCCA) sensors for Ni2+ may be useful in developing a point-of-care test to determine metal binding affinity in plasma and to help rule out myocardial ischemia. PCCA sensors for Ni2+, with 5-amino-8-hydroxyquinoline as a chelating agent, were tested in aqueous solutions and diluted human plasma. The peak wavelength diffracted by the sensors was monitored by reflectance spectrometry and correlated with Ni2+ concentration. The PCCA sensors show a linear response to aqueous Ni2+ concentrations between 0.2 and 1.0 mmol L-1, and can detect changes in free Ni2+ concentration of <60 μmol L-1. The sensors respond at physiologic pH and can be reversibly dehydrated. The PCCA sensors developed here can report on free Ni2+ concentration in the presence of human plasma. These sensors can be used to detect a decrease in the Ni2+ affinity of plasma proteins, which may indicate recent myocardial ischemia. PCCA sensors offer a practical approach to rapid, point-of-care detection of a proposed biochemical signature of myocardial ischemia. © The Royal Society of Chemistry.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Baca, JT
Finegold, DN
Asher, SAasher@pitt.eduASHER
Date: 1 January 2008
Date Type: Publication
Journal or Publication Title: Analyst
Volume: 133
Number: 3
Page Range: 385 - 390
DOI or Unique Handle: 10.1039/b712482a
Schools and Programs: Dietrich School of Arts and Sciences > Chemistry
Refereed: Yes
ISSN: 0003-2654
MeSH Headings: Biological Markers--blood; Colloids; Crystallization; Equipment Design; Humans; Hydrogen-Ion Concentration; Myocardial Ischemia--diagnosis; Nickel--blood; Point-of-Care Systems
PubMed ID: 18299754
Date Deposited: 21 Feb 2013 22:36
Last Modified: 22 Jun 2021 11:55


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