Baca, JT and Finegold, DN and Asher, SA
(2008)
Progress in developing polymerized crystalline colloidal array sensors for point-of-care detection of myocardial ischemia.
Analyst, 133 (3).
385 - 390.
ISSN 0003-2654
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Abstract
The difficulty of rapid, definitive diagnosis of myocardial ischemia leads to unnecessary hospital admissions and treatment delays. Previously, decreased metal binding affinity in human serum was investigated as a marker for myocardial ischemia. Polymerized Crystalline Colloidal Array (PCCA) sensors for Ni2+ may be useful in developing a point-of-care test to determine metal binding affinity in plasma and to help rule out myocardial ischemia. PCCA sensors for Ni2+, with 5-amino-8-hydroxyquinoline as a chelating agent, were tested in aqueous solutions and diluted human plasma. The peak wavelength diffracted by the sensors was monitored by reflectance spectrometry and correlated with Ni2+ concentration. The PCCA sensors show a linear response to aqueous Ni2+ concentrations between 0.2 and 1.0 mmol L-1, and can detect changes in free Ni2+ concentration of <60 μmol L-1. The sensors respond at physiologic pH and can be reversibly dehydrated. The PCCA sensors developed here can report on free Ni2+ concentration in the presence of human plasma. These sensors can be used to detect a decrease in the Ni2+ affinity of plasma proteins, which may indicate recent myocardial ischemia. PCCA sensors offer a practical approach to rapid, point-of-care detection of a proposed biochemical signature of myocardial ischemia. © The Royal Society of Chemistry.
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Details
Item Type: |
Article
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Status: |
Published |
Creators/Authors: |
Creators | Email | Pitt Username | ORCID  |
---|
Baca, JT | | | | Finegold, DN | | | | Asher, SA | asher@pitt.edu | ASHER | |
|
Date: |
1 January 2008 |
Date Type: |
Publication |
Journal or Publication Title: |
Analyst |
Volume: |
133 |
Number: |
3 |
Page Range: |
385 - 390 |
DOI or Unique Handle: |
10.1039/b712482a |
Schools and Programs: |
Dietrich School of Arts and Sciences > Chemistry |
Refereed: |
Yes |
ISSN: |
0003-2654 |
MeSH Headings: |
Biological Markers--blood; Colloids; Crystallization; Equipment Design; Humans; Hydrogen-Ion Concentration; Myocardial Ischemia--diagnosis; Nickel--blood; Point-of-Care Systems |
PubMed ID: |
18299754 |
Date Deposited: |
21 Feb 2013 22:36 |
Last Modified: |
22 Jun 2021 11:55 |
URI: |
http://d-scholarship.pitt.edu/id/eprint/17336 |
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