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Assignments and conformational dependencies of the amide III peptide backbone UV resonance Raman bands

Mikhonin, AV and Ahmed, Z and Ianoul, A and Asher, SA (2004) Assignments and conformational dependencies of the amide III peptide backbone UV resonance Raman bands. Journal of Physical Chemistry B, 108 (49). 19020 - 19028. ISSN 1520-6106

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We investigated the assignments and the conformational dependencies of the UV resonance Raman bands of the 21-residue mainly alanine peptide (AP) and its isotopically substituted derivatives in both their α-helical and PPII states. We also examined smaller peptides to correlate conformation, hydrogen bonding, and structure. Our vibrational mode analysis confirms the complex nature of the amide III region, which contains many vibrational modes. We assign these bands by interpreting the isotopically induced frequency shifts and the conformational sensitivity of these bands and their temperature dependence. Our assignments of the amide bands in some cases agree, but in other cases challenge previous assignments by Lee and Krimm (Biopolymers 1998, 46, 283-317), Overman and Thomas (Biochemistry 1998, 37, 5654-5665), and Diem et al. (J. Phys. Chem. 1992, 96, 548-554). We see evidence for the partial dehydration of α-helices at elevated temperatures.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Mikhonin, AV
Ahmed, Z
Ianoul, A
Asher, SAasher@pitt.eduASHER
Date: 9 December 2004
Date Type: Publication
Journal or Publication Title: Journal of Physical Chemistry B
Volume: 108
Number: 49
Page Range: 19020 - 19028
DOI or Unique Handle: 10.1021/jp045959d
Schools and Programs: Dietrich School of Arts and Sciences > Chemistry
Refereed: Yes
ISSN: 1520-6106
Date Deposited: 27 Feb 2013 18:35
Last Modified: 02 Feb 2019 16:55


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