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Transcriptional silencing by the mycobacteriophage L5 repressor

Brown, KL and Sarkis, GJ and Wadsworth, C and Hatfull, GF (1997) Transcriptional silencing by the mycobacteriophage L5 repressor. EMBO Journal, 16 (19). 5914 - 5921. ISSN 0261-4189

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The success of a temperate bacteriophage is dependent upon its ability to completely shut down expression of its lytic genes during lysogenic growth. Mycobacteriophage L5 accomplishes this by an atypical phage repressor, gp71, which binds to multiple asymmetric DNA sites. L5 gp71 regulates transcription initiation at an early lytic promoter, P(left), but also affects downstream gene expression at 'stoperator' sites in the phage genome. The L5 genome is replete with stoperator sites located within short intergenic spaces in both the early and late lytic operons and oriented specifically with respect to transcription. Binding of gp71 to these sites results in a strong orientation-dependent polar effect on downstream gene expression and global silencing of prophage gene expression.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Brown, KL
Sarkis, GJ
Wadsworth, C
Hatfull, GFgfh@pitt.eduGFH
Date: 18 October 1997
Date Type: Publication
Journal or Publication Title: EMBO Journal
Volume: 16
Number: 19
Page Range: 5914 - 5921
DOI or Unique Handle: 10.1093/emboj/16.19.5914
Schools and Programs: Dietrich School of Arts and Sciences > Biological Sciences
Refereed: Yes
ISSN: 0261-4189
MeSH Headings: Base Sequence; Binding Sites; DNA Footprinting; DNA, Viral--metabolism; DNA-Binding Proteins--metabolism; Gene Expression Regulation, Viral; Molecular Sequence Data; Mycobacteriophages--genetics; Mycobacteriophages--metabolism; Promoter Regions, Genetic; Repressor Proteins--metabolism; Transcription, Genetic; Viral Proteins--metabolism
Other ID: NLM PMC1170222
PubMed Central ID: PMC1170222
PubMed ID: 9312049
Date Deposited: 01 Mar 2013 15:32
Last Modified: 02 Feb 2019 15:57


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