Brown, KL and Sarkis, GJ and Wadsworth, C and Hatfull, GF
(1997)
Transcriptional silencing by the mycobacteriophage L5 repressor.
EMBO Journal, 16 (19).
5914 - 5921.
ISSN 0261-4189
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Abstract
The success of a temperate bacteriophage is dependent upon its ability to completely shut down expression of its lytic genes during lysogenic growth. Mycobacteriophage L5 accomplishes this by an atypical phage repressor, gp71, which binds to multiple asymmetric DNA sites. L5 gp71 regulates transcription initiation at an early lytic promoter, P(left), but also affects downstream gene expression at 'stoperator' sites in the phage genome. The L5 genome is replete with stoperator sites located within short intergenic spaces in both the early and late lytic operons and oriented specifically with respect to transcription. Binding of gp71 to these sites results in a strong orientation-dependent polar effect on downstream gene expression and global silencing of prophage gene expression.
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Details
Item Type: |
Article
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Status: |
Published |
Creators/Authors: |
Creators | Email | Pitt Username | ORCID  |
---|
Brown, KL | | | | Sarkis, GJ | | | | Wadsworth, C | | | | Hatfull, GF | gfh@pitt.edu | GFH | |
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Date: |
18 October 1997 |
Date Type: |
Publication |
Journal or Publication Title: |
EMBO Journal |
Volume: |
16 |
Number: |
19 |
Page Range: |
5914 - 5921 |
DOI or Unique Handle: |
10.1093/emboj/16.19.5914 |
Schools and Programs: |
Dietrich School of Arts and Sciences > Biological Sciences |
Refereed: |
Yes |
ISSN: |
0261-4189 |
MeSH Headings: |
Base Sequence; Binding Sites; DNA Footprinting; DNA, Viral--metabolism; DNA-Binding Proteins--metabolism; Gene Expression Regulation, Viral; Molecular Sequence Data; Mycobacteriophages--genetics; Mycobacteriophages--metabolism; Promoter Regions, Genetic; Repressor Proteins--metabolism; Transcription, Genetic; Viral Proteins--metabolism |
Other ID: |
NLM PMC1170222 |
PubMed Central ID: |
PMC1170222 |
PubMed ID: |
9312049 |
Date Deposited: |
01 Mar 2013 15:32 |
Last Modified: |
02 Feb 2019 15:57 |
URI: |
http://d-scholarship.pitt.edu/id/eprint/17562 |
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