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Mycobacteriophage 15 infection of Mycobacterium bovis BCG: Implications for phage genetics in the slow-growing mycobacteria

Fullner, KJ and Hatfull, GF (1997) Mycobacteriophage 15 infection of Mycobacterium bovis BCG: Implications for phage genetics in the slow-growing mycobacteria. Molecular Microbiology, 26 (4). 755 - 766. ISSN 0950-382X

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Mycobacteriophage L5 is a well-characterized temperate phage that forms stable lysogens in Mycobacterium smegmatis. The host range of L5 is, however, unclear because previous reports suggested that it does not infect stow-growing mycobacteria such as Mycobacterium tuberculosis and bacille Calmette-Guerin (BCG). Moreover, luciferase reporter phage derivatives of L5 failed to produce light from BCG, suggesting that infection is blocked at or before the stage of DNA injection. In this study, we demonstrate that L5 infection of slow growing mycobacteria specifically requires a high concentration of Ca2+, conditions that differs from those required for infection of M. smegmatis by L5 and for infection of BCG by the closely related phage D29. In addition, we show that there are specific genetic determinants of L5 that confer the ability to infect slow growing mycobacteria, without altering infection of M. smegmatis. These observations extend the use of phage L5 for the diagnosis and analysis of tuberculosis and other mycobacterial diseases.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Fullner, KJ
Hatfull, GFgfh@pitt.eduGFH
Date: 1 January 1997
Date Type: Publication
Journal or Publication Title: Molecular Microbiology
Volume: 26
Number: 4
Page Range: 755 - 766
DOI or Unique Handle: 10.1046/j.1365-2958.1997.6111984.x
Schools and Programs: Dietrich School of Arts and Sciences > Biological Sciences
Refereed: Yes
ISSN: 0950-382X
MeSH Headings: Calcium--metabolism; Calcium--pharmacology; Cations, Divalent; Luciferases--genetics; Luciferases--metabolism; Lysogeny; Mutation; Mycobacteriophages--genetics; Mycobacteriophages--physiology; Mycobacterium--growth & development; Mycobacterium--virology; Mycobacterium bovis--growth & development; Mycobacterium bovis--virology
PubMed ID: 9427405
Date Deposited: 01 Mar 2013 15:29
Last Modified: 22 Jun 2021 12:56


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