Hughes, RE and Hatfull, GF and Rice, P and Steitz, TA and Grindley, NDF
(1990)
Cooperativity mutants of the γδ resolvase identify an essential interdimer interaction.
Cell, 63 (6).
1331 - 1338.
ISSN 0092-8674
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Abstract
γδ resolvase, a transposon-encoded site-specific recombinase, catalyzes the resolution of the cointegrate intermediate of γδ transposition. The recombination reaction involves the formation of a catalytic nucleoprotein complex whose structure is determined by specific protein-DNA and protein-protein interactions. We have isolated many resolvase mutants and have identified four that are unable to mediate a subclass of higher order protein-protein interactions necessary for recombination. This mutant phenotype is characterized by an inability to catalyze recombination, a loss of cooperative binding to res DNA, and a failure to induce looping out of the DNA between two resolvase binding sites within res. The amino acid side chains identified by the cooperativity mutants cluster on a surface of the protein that mediates an interaction between resolvase dimers in a crystallographic tetramer. We have therefore identified a region of resolvase that mediates an interdimer protein-protein interaction necessary for the formation of the recombinogenic synaptic intermediate. © 1990.
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Details
Item Type: |
Article
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Status: |
Published |
Creators/Authors: |
Creators | Email | Pitt Username | ORCID  |
---|
Hughes, RE | | | | Hatfull, GF | gfh@pitt.edu | GFH | | Rice, P | | | | Steitz, TA | | | | Grindley, NDF | | | |
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Date: |
21 December 1990 |
Date Type: |
Publication |
Journal or Publication Title: |
Cell |
Volume: |
63 |
Number: |
6 |
Page Range: |
1331 - 1338 |
DOI or Unique Handle: |
10.1016/0092-8674(90)90428-h |
Schools and Programs: |
Dietrich School of Arts and Sciences > Biological Sciences |
Refereed: |
Yes |
ISSN: |
0092-8674 |
MeSH Headings: |
Amino Acid Sequence; Binding Sites; DNA Transposable Elements; Macromolecular Substances; Models, Molecular; Molecular Sequence Data; Mutagenesis; Nucleotidyltransferases--genetics; Nucleotidyltransferases--metabolism; Plasmids; Protein Conformation; Recombination, Genetic; Transposases |
PubMed ID: |
2175679 |
Date Deposited: |
01 Mar 2013 15:28 |
Last Modified: |
02 Feb 2019 15:57 |
URI: |
http://d-scholarship.pitt.edu/id/eprint/17580 |
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