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Cooperativity mutants of the γδ resolvase identify an essential interdimer interaction

Hughes, RE and Hatfull, GF and Rice, P and Steitz, TA and Grindley, NDF (1990) Cooperativity mutants of the γδ resolvase identify an essential interdimer interaction. Cell, 63 (6). 1331 - 1338. ISSN 0092-8674

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Abstract

γδ resolvase, a transposon-encoded site-specific recombinase, catalyzes the resolution of the cointegrate intermediate of γδ transposition. The recombination reaction involves the formation of a catalytic nucleoprotein complex whose structure is determined by specific protein-DNA and protein-protein interactions. We have isolated many resolvase mutants and have identified four that are unable to mediate a subclass of higher order protein-protein interactions necessary for recombination. This mutant phenotype is characterized by an inability to catalyze recombination, a loss of cooperative binding to res DNA, and a failure to induce looping out of the DNA between two resolvase binding sites within res. The amino acid side chains identified by the cooperativity mutants cluster on a surface of the protein that mediates an interaction between resolvase dimers in a crystallographic tetramer. We have therefore identified a region of resolvase that mediates an interdimer protein-protein interaction necessary for the formation of the recombinogenic synaptic intermediate. © 1990.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Hughes, RE
Hatfull, GFgfh@pitt.eduGFH
Rice, P
Steitz, TA
Grindley, NDF
Date: 21 December 1990
Date Type: Publication
Journal or Publication Title: Cell
Volume: 63
Number: 6
Page Range: 1331 - 1338
DOI or Unique Handle: 10.1016/0092-8674(90)90428-h
Schools and Programs: Dietrich School of Arts and Sciences > Biological Sciences
Refereed: Yes
ISSN: 0092-8674
MeSH Headings: Amino Acid Sequence; Binding Sites; DNA Transposable Elements; Macromolecular Substances; Models, Molecular; Molecular Sequence Data; Mutagenesis; Nucleotidyltransferases--genetics; Nucleotidyltransferases--metabolism; Plasmids; Protein Conformation; Recombination, Genetic; Transposases
PubMed ID: 2175679
Date Deposited: 01 Mar 2013 15:28
Last Modified: 02 Feb 2019 15:57
URI: http://d-scholarship.pitt.edu/id/eprint/17580

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