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Superinfection immunity of mycobacteriophage L5: applications for genetic transformation of mycobacteria

Donnelly‐Wu, MK and Jacobs, WR and Hatfull, GF (1993) Superinfection immunity of mycobacteriophage L5: applications for genetic transformation of mycobacteria. Molecular Microbiology, 7 (3). 407 - 417. ISSN 0950-382X

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Mycobacteriophage L5 is a temperate phage of the mycobacteria that forms stable lysogens in Mycobacterium smegmatis. We show here that the 183‐amino‐acid product of L5 gene 71 confers immunity to L5 superinfection, is required for maintenance of the lysogenic state and contains a helix‐turn‐helix DNA‐binding motif—properties associated with repressors of temperate phages. We have utilized these observations to demonstrate the use of L5 gene 71 as a selectable marker for genetic transformation of the mycobacteria. Significantly, the use of L5 gene 71 as a selectable gene avoids the requirement for antibiotic‐resistance genes providing an important tool for manipulation of the pathogens Mycobacterium tuberculosis and Mycobacterium avium, and for the construction of recombinant BCG vaccines. Copyright © 1993, Wiley Blackwell. All rights reserved


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Donnelly‐Wu, MK
Jacobs, WR
Hatfull, GFgfh@pitt.eduGFH
Date: 1 January 1993
Date Type: Publication
Journal or Publication Title: Molecular Microbiology
Volume: 7
Number: 3
Page Range: 407 - 417
DOI or Unique Handle: 10.1111/j.1365-2958.1993.tb01132.x
Schools and Programs: Dietrich School of Arts and Sciences > Biological Sciences
Refereed: Yes
ISSN: 0950-382X
MeSH Headings: Amino Acid Sequence; Base Sequence; DNA Mutational Analysis; DNA, Bacterial--genetics; DNA, Recombinant--genetics; DNA-Binding Proteins--genetics; Genes, Viral--genetics; Genetic Markers; Hot Temperature; Lysogeny--genetics; Molecular Sequence Data; Mycobacteriaceae--genetics; Mycobacteriophages--genetics; Mycobacteriophages--growth & development; Phenotype; Plasmids--genetics; Restriction Mapping; Transformation, Genetic; Viral Plaque Assay; Viral Proteins--genetics
PubMed ID: 8459767
Date Deposited: 01 Mar 2013 19:10
Last Modified: 03 Aug 2020 14:55


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