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Characterization of a Mycobacterium smegmatis gene that confers resistance to phages L5 and D29 when overexpressed

Barsom, EK and Hatfull, GF (1996) Characterization of a Mycobacterium smegmatis gene that confers resistance to phages L5 and D29 when overexpressed. Molecular Microbiology, 21 (1). 159 - 170. ISSN 0950-382X

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Bacteriophage infection requires a specific interaction with the outer surface of a bacterial host followed by interaction with the cell membrane and phage DNA injection. Phages of the mycobacteria encounter a cell wall that is rich in unusual lipid- and sugar-containing components which form a formidable barrier that must be passed to gain access to the membrane. We describe here a gene of Mycobacterium smegmatis that confers resistance to mycobacteriophages L5 and D29. The phage-resistance phenotype results not from mutation but from elevated expression of a wild-type gene. It appears that the product of this multicopy phage-resistance (mpr) gene may alter the structure of the host cell wall or membrane, thereby inhibiting productive phage DNA injection.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Barsom, EK
Hatfull, GFgfh@pitt.eduGFH
Date: 1 January 1996
Date Type: Publication
Journal or Publication Title: Molecular Microbiology
Volume: 21
Number: 1
Page Range: 159 - 170
DOI or Unique Handle: 10.1046/j.1365-2958.1996.6291342.x
Schools and Programs: Dietrich School of Arts and Sciences > Biological Sciences
Refereed: Yes
ISSN: 0950-382X
MeSH Headings: Amino Acid Sequence; Base Sequence; Cell Membrane--virology; Cell Wall--virology; Chromosome Mapping; DNA, Bacterial--genetics; Escherichia coli--genetics; Gene Expression; Genes, Bacterial; Genes, Reporter; Luciferases--genetics; Molecular Sequence Data; Mutation; Mycobacteriophages--genetics; Mycobacteriophages--pathogenicity; Mycobacteriophages--physiology; Mycobacterium--genetics; Mycobacterium--virology; Open Reading Frames; Plasmids--genetics; Recombination, Genetic; Transfection; Transformation, Genetic
PubMed ID: 8843442
Date Deposited: 01 Mar 2013 19:09
Last Modified: 02 Feb 2019 15:57


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