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Mycobacteriophage TM4: Genome structure and gene expression

Ford, ME and Stenstrom, C and Hendrix, RW and Hatfull, GF (1998) Mycobacteriophage TM4: Genome structure and gene expression. Tubercle and Lung Disease, 79 (2). 63 - 73. ISSN 0962-8479

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Mycobacteriophage TM4 is a dsDNA-tailed phage that infects both fast-growing and slow-growing strains of mycobacteria. While TM4 has been used extensively for the construction of mycobacterial shuttle phasmids and for the delivery of reporter genes and transposons into mycobacterial cells, little is known about its genetics or molecular biology. We describe here the complete 52,797 bp genome sequence of TM4 and a map of its genome organization. While not a close relative of other mycobacteriophages, TM4 encodes several proteins with sequence similarity to those of other bacteriophages - including L5 and D29 - indicating that they have common ancestry. In addition, TM4 encodes proteins with similarity to haloperoxidases, glutaredoxins and the WhiB family of transcriptional regulators. Following infection, TM4 genes are expressed in a defined temporal pattern, with the virion structural proteins expressed late in the phage growth cycle. Understanding the genetics of TM4 will greatly facilitate its use as a tool for the genetic manipulation of the mycobacteria.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Ford, ME
Stenstrom, C
Hendrix, RWrhx@pitt.eduRHX
Hatfull, GFgfh@pitt.eduGFH
Date: 1 January 1998
Date Type: Publication
Journal or Publication Title: Tubercle and Lung Disease
Volume: 79
Number: 2
Page Range: 63 - 73
DOI or Unique Handle: 10.1054/tuld.1998.0007
Schools and Programs: Dietrich School of Arts and Sciences > Biological Sciences
Refereed: Yes
ISSN: 0962-8479
MeSH Headings: Bacteriophage Typing; Base Sequence; Electrophoresis, Polyacrylamide Gel; Gene Expression Regulation, Bacterial; Genes, Viral--genetics; Molecular Sequence Data; Mycobacteriophages--chemistry; Mycobacteriophages--genetics; Mycobacterium smegmatis--metabolism; Sequence Alignment; Sequence Analysis, DNA; Sequence Analysis, Protein; Viral Proteins--analysis; Viral Proteins--metabolism
PubMed ID: 10645443
Date Deposited: 01 Mar 2013 20:43
Last Modified: 02 Feb 2019 15:57


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