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DNA sequence, structure and gene expression of mycobacteriophage L5: a phage system for mycobacterial genetics

Hatfult, GF and Sarkis, GJ (1993) DNA sequence, structure and gene expression of mycobacteriophage L5: a phage system for mycobacterial genetics. Molecular Microbiology, 7 (3). 395 - 405. ISSN 0950-382X

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Abstract

Genetic studies of Mycobacterium tuberculosis and other mycobacterial pathogens have suffered from the lack of a sophisticated genetic system. To address this issue we have developed a viral system through a detailed characterization of mycobacteriophage L5, a temperate phage that infects both fast‐ and slow‐growing mycobacteria. We describe here the complete DNA sequence of the L5 genome and initial characterization of L5 virion structure and gene expression. In addition to providing a genetic‘toolbox’for the mycobacteria we find that L5 offers a new paradigm for dsDNA phages, being phenotypically temperate but employing genetic strategies for phage growth usually associated with lytic bacteriophages. Copyright © 1993, Wiley Blackwell. All rights reserved

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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Hatfult, GF
Sarkis, GJ
Date: 1 January 1993
Date Type: Publication
Journal or Publication Title: Molecular Microbiology
Volume: 7
Number: 3
Page Range: 395 - 405
DOI or Unique Handle: 10.1111/j.1365-2958.1993.tb01131.x
Schools and Programs: Dietrich School of Arts and Sciences > Biological Sciences
Refereed: Yes
ISSN: 0950-382X
MeSH Headings: Amino Acid Sequence; Bacteriophages--classification; Base Sequence; Codon--genetics; DNA, Viral--genetics; Gene Expression; Genes, Viral--genetics; Genome, Viral; Molecular Sequence Data; Mycobacteriaceae--genetics; Mycobacteriophages--classification; Mycobacteriophages--genetics; Mycobacteriophages--ultrastructure; Viral Structural Proteins--genetics; Virion--genetics
PubMed ID: 8459766
Date Deposited: 01 Mar 2013 20:43
Last Modified: 02 Feb 2019 15:57
URI: http://d-scholarship.pitt.edu/id/eprint/17605

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