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Rapid assessment of drug susceptibilities of Mycobacterium tuberculosis by means of luciferase reporter phages

Jacobs, WR and Barletta, RG and Udani, R and Chan, J and Kalkut, G and Sosne, G and Kieser, T and Sarkis, GJ and Hatfull, GF and Bloom, BR (1993) Rapid assessment of drug susceptibilities of Mycobacterium tuberculosis by means of luciferase reporter phages. Science, 260 (5109). 819 - 822. ISSN 0036-8075

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Abstract

Effective chemotherapy of tuberculosis requires rapid assessment of drug sensitivity because of the emergence of multidrug-resistant Mycobacterium tuberculosis. Drug susceptibility was assessed by a simple method based on the efficient production of photons by viable mycobacteria infected with specific reporter phages expressing the firefly luciferase gene. Light production was dependent on phage infection, expression of the luciferase gene, and the level of cellular adenosine triphosphate. Signals could be detected within minutes after infection of virulent M. tuberculosis with reporter phages. Culture of conventional strains with antituberculosis drugs, including isoniazid or rifampicin, resulted in extinction of light production. In contrast, light signals after luciferase reporter phage infection of drug-resistant strains continued to be produced. Luciferase reporter phages may help to reduce the time required for establishing antibiotic sensitivity of M. tuberculosis strains from weeks to days and to accelerate screening for new antituberculosis drugs.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Jacobs, WR
Barletta, RG
Udani, R
Chan, J
Kalkut, G
Sosne, G
Kieser, T
Sarkis, GJ
Hatfull, GFgfh@pitt.eduGFH
Bloom, BR
Date: 1 January 1993
Date Type: Publication
Journal or Publication Title: Science
Volume: 260
Number: 5109
Page Range: 819 - 822
DOI or Unique Handle: 10.1126/science.8484123
Schools and Programs: Dietrich School of Arts and Sciences > Biological Sciences
Refereed: Yes
ISSN: 0036-8075
MeSH Headings: Adenosine Triphosphate--metabolism; Antitubercular Agents--pharmacology; Cloning, Molecular; Drug Resistance, Microbial; Luciferases--genetics; Luciferases--metabolism; Luminescent Measurements; Microbial Sensitivity Tests--methods; Mycobacteriophages--genetics; Mycobacterium--genetics; Mycobacterium--metabolism; Mycobacterium bovis--drug effects; Mycobacterium bovis--genetics; Mycobacterium bovis--metabolism; Mycobacterium tuberculosis--drug effects; Mycobacterium tuberculosis--genetics; Mycobacterium tuberculosis--metabolism
PubMed ID: 8484123
Date Deposited: 01 Mar 2013 20:43
Last Modified: 02 Feb 2019 15:57
URI: http://d-scholarship.pitt.edu/id/eprint/17606

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