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Mycobacteriophage L5 integrase-mediated site-specific integration in vitro

Mong Hong Lee, and Hatfull, GF (1993) Mycobacteriophage L5 integrase-mediated site-specific integration in vitro. Journal of Bacteriology, 175 (21). 6836 - 6841. ISSN 0021-9193

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Mycobacteriophage L5, a temperate phage of the mycobacteria, forms stable lysogens in Mycobacterium smegmatis via site-specific integration of the phage genome. Recombination occurs within specific phage and bacterial attachment sites and is catalyzed by the phage-encoded integrase protein in vivo. We describe here the overexpression and purification of L5 integrase and its ability to mediate integrative recombination in vitro. We find that L5 integrase-mediated recombination is greatly stimulated by extracts of M. smegmatis but not by Escherichia coli extracts, purified E. coli integration host factor, or purified HU, indicating the presence of a novel mycobacterial integration host factor.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Mong Hong Lee,
Hatfull, GFgfh@pitt.eduGFH
Date: 1 January 1993
Date Type: Publication
Journal or Publication Title: Journal of Bacteriology
Volume: 175
Number: 21
Page Range: 6836 - 6841
DOI or Unique Handle: 10.1128/jb.175.21.6836-6841.1993
Schools and Programs: Dietrich School of Arts and Sciences > Biological Sciences
Refereed: Yes
ISSN: 0021-9193
MeSH Headings: Amino Acid Sequence; Base Sequence; DNA Nucleotidyltransferases--biosynthesis; DNA Nucleotidyltransferases--isolation & purification; DNA Nucleotidyltransferases--metabolism; DNA Primers; Integrases; Kinetics; Molecular Sequence Data; Mycobacteriophages--enzymology; Mycobacteriophages--genetics; Mycobacterium--enzymology; Mycobacterium--genetics; Plasmids; Recombinant Proteins--biosynthesis; Recombinant Proteins--isolation & purification; Recombinant Proteins--metabolism; Recombination, Genetic; Restriction Mapping; Thermodynamics; Virus Integration
Other ID: NLM PMC206807
PubMed Central ID: PMC206807
PubMed ID: 8226625
Date Deposited: 12 Mar 2013 19:02
Last Modified: 30 Sep 2022 04:55


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