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A novel host factor for integration of mycobacteriophage L5

Pedulla, ML and Lee, MH and Lever, DC and Hatfull, GF (1996) A novel host factor for integration of mycobacteriophage L5. Proceedings of the National Academy of Sciences of the United States of America, 93 (26). 15411 - 15416. ISSN 0027-8424

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Bacterial integration host factors (IHFs) play central roles in the cellular processes of recombination, DNA replication, transcription, and bacterial pathogenesis. We describe here a novel mycobacterial IHF (mIHF) of Mycobacterium smegmatis and Mycobacterium tuberculosis that stimulates integration of mycobacteriophage L5. mIHF is the product of a single gene and is unrelated at the sequence level to other integration host factors. By itself, mIHF does not bind preferentially to attP DNA, although it significantly alters the pattern of integrase (Int) binding, promoting the formation of specific integrase-mIHF-attP intasome complexes.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Pedulla, ML
Lee, MH
Lever, DC
Hatfull, GFgfh@pitt.eduGFH
Date: 24 December 1996
Date Type: Publication
Journal or Publication Title: Proceedings of the National Academy of Sciences of the United States of America
Volume: 93
Number: 26
Page Range: 15411 - 15416
DOI or Unique Handle: 10.1073/pnas.93.26.15411
Schools and Programs: Dietrich School of Arts and Sciences > Biological Sciences
Refereed: Yes
ISSN: 0027-8424
MeSH Headings: Amino Acid Sequence; Bacterial Proteins--biosynthesis; Bacterial Proteins--chemistry; Bacterial Proteins--isolation & purification; Base Sequence; DNA Footprinting; DNA-Binding Proteins--biosynthesis; Deoxyribonuclease I; Genes, Viral; Integration Host Factors; Molecular Sequence Data; Mycobacteriophages--genetics; Mycobacteriophages--physiology; Mycobacterium--virology; Mycobacterium tuberculosis--virology; Recombination, Genetic; Sequence Homology, Amino Acid
Other ID: NLM PMC26418
PubMed Central ID: PMC26418
PubMed ID: 8986825
Date Deposited: 12 Mar 2013 19:08
Last Modified: 02 Feb 2019 15:57


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