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The role of supercoiling in mycobacteriophage L5 integrative recombination

Peña, CEA and Kahlenberg, JM and Hatfull, GF (1998) The role of supercoiling in mycobacteriophage L5 integrative recombination. Nucleic Acids Research, 26 (17). 4012 - 4018. ISSN 0305-1048

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Abstract

The genome of temperate mycobacteriophage L5 integrates into the chromosomes of its hosts, including Mycobacterium smegmatis, Mycobacterium tuberculosis and bacille Calmette-Guerin. This integrase-mediated site-specific recombination reaction occurs between the phage attP site and the mycobacterial attB site and requires the mycobacterial integration host factor. Here we examine the role of supercoiling in this reaction and show that integration is stimulated by DNA supercoiling but that supercoiling of either the attP or the attB substrate enhances recombination. Supercoiling thus facilitates a postsynaptic recombination event. We also show that, while supercoiling is not required for the production of a recombinagenic intasome, a mutant attP DNA deficient in binding of the host factor acquires a dependence on supercoiling for intasome formation and recombination.

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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Peña, CEA
Kahlenberg, JM
Hatfull, GFgfh@pitt.eduGFH
Date: 1 September 1998
Date Type: Publication
Journal or Publication Title: Nucleic Acids Research
Volume: 26
Number: 17
Page Range: 4012 - 4018
DOI or Unique Handle: 10.1093/nar/26.17.4012
Schools and Programs: Dietrich School of Arts and Sciences > Biological Sciences
Refereed: Yes
ISSN: 0305-1048
MeSH Headings: Bacterial Proteins--metabolism; Bacteriophage lambda--genetics; Base Sequence; DNA Topoisomerases, Type I--metabolism; DNA, Superhelical--genetics; DNA, Superhelical--metabolism; DNA, Viral--genetics; DNA, Viral--metabolism; Integration Host Factors; Molecular Sequence Data; Mycobacteriophages--genetics; Nucleic Acid Conformation; Recombination, Genetic; Virus Integration--genetics
Other ID: NLM PMC147811
PubMed Central ID: PMC147811
PubMed ID: 9705513
Date Deposited: 12 Mar 2013 19:08
Last Modified: 28 Sep 2022 14:00
URI: http://d-scholarship.pitt.edu/id/eprint/17642

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