Peña, CEA and Lee, MH and Pedulla, ML and Hatfull, GF
(1997)
Characterization of the Mycobacteriophage L5 attachment site, attP.
Journal of Molecular Biology, 266 (1).
76 - 92.
ISSN 0022-2836
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Abstract
Lysogenization of mycobacteriophage L5 involves integration of the phage genome into the Mycobacterium smegmatis chromosome. Integration occurs by a site-specific recombination event between a phage attachment site, attP, and a bacterial attachment site, attB, which is catalyzed by the phage-encoded integrase protein. DNase I footprinting reveals that L5 integrase binds to two types of sites within attP which span an unexpectedly large region of 413 bp: seven arm-type sites (P1 to P7) each of which correspond to a consensus sequence 5'-TGCaaCtcYy, and core-type sites at the points of strand exchange. Mutational analyses indicate that not all of the arm-type sites are required for integration, and that the P3 site and the rightmost pair of sites (P6 and P7) are dispensable for Integration. We show that a 252 bp segment of attP DNA is sufficient for efficient integrative recombination and that int can be provided in trans for simple and efficient transformation of the mycobacteria.
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Details
Item Type: |
Article
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Status: |
Published |
Creators/Authors: |
Creators | Email | Pitt Username | ORCID  |
---|
Peña, CEA | | | | Lee, MH | | | | Pedulla, ML | | | | Hatfull, GF | gfh@pitt.edu | GFH | |
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Date: |
14 February 1997 |
Date Type: |
Publication |
Journal or Publication Title: |
Journal of Molecular Biology |
Volume: |
266 |
Number: |
1 |
Page Range: |
76 - 92 |
DOI or Unique Handle: |
10.1006/jmbi.1996.0774 |
Schools and Programs: |
Dietrich School of Arts and Sciences > Biological Sciences |
Refereed: |
Yes |
ISSN: |
0022-2836 |
MeSH Headings: |
Bacteriophages--genetics; Bacteriophages--physiology; Base Sequence; Binding Sites; Chromosomes, Bacterial; Cloning, Molecular; Consensus Sequence; DNA Footprinting; DNA Mutational Analysis; Deoxyribonuclease I; Escherichia coli; Genome, Viral; Integrases--metabolism; Molecular Sequence Data; Mycobacterium--genetics; Mycobacterium--virology; Recombination, Genetic; Sequence Deletion; Virus Integration |
PubMed ID: |
9054972 |
Date Deposited: |
12 Mar 2013 19:09 |
Last Modified: |
02 Feb 2019 15:57 |
URI: |
http://d-scholarship.pitt.edu/id/eprint/17643 |
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