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Positions of strand exchange in mycobacteriophage L5 integration and characterization of the attB site

Peña, CEA and Stoner, JE and Hatfull, GF (1996) Positions of strand exchange in mycobacteriophage L5 integration and characterization of the attB site. Journal of Bacteriology, 178 (18). 5533 - 5536. ISSN 0021-9193

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Abstract

Mycobacteriophage L5 integrates into the genome of Mycobacterium smegmatis via site-specific recombination between the phage attP site and the bacterial attB site. These two sites have a 43-bp common core sequence within which strand exchange occurs and which overlaps a tRNA(Gly) gene at attB. We show here that a 29-bp segment of DNA is necessary and sufficient for attB function and identify the positions of strand exchange.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Peña, CEA
Stoner, JE
Hatfull, GFgfh@pitt.eduGFH
Date: 1 January 1996
Date Type: Publication
Journal or Publication Title: Journal of Bacteriology
Volume: 178
Number: 18
Page Range: 5533 - 5536
DOI or Unique Handle: 10.1128/jb.178.18.5533-5536.1996
Schools and Programs: Dietrich School of Arts and Sciences > Biological Sciences
Refereed: Yes
ISSN: 0021-9193
MeSH Headings: Anticodon; Base Sequence; DNA, Bacterial--genetics; Molecular Sequence Data; Mycobacteriophages--genetics; RNA, Transfer--genetics; Recombination, Genetic; Virus Integration--genetics
Other ID: NLM PMC178380
PubMed Central ID: PMC178380
PubMed ID: 8808947
Date Deposited: 12 Mar 2013 19:09
Last Modified: 02 Feb 2019 15:57
URI: http://d-scholarship.pitt.edu/id/eprint/17645

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