Peña, CEA and Kahlenberg, JM and Hatfull, GF
(1999)
Protein-DNA complexes in mycobacteriophage L5 integrative recombination.
Journal of Bacteriology, 181 (2).
454 - 461.
ISSN 0021-9193
Abstract
The temperate mycobacteriophage L5 integrates site specifically into the genomes of Mycobacterium smegmatis, Mycobacterium tuberculosis, and Mycobacterium bovis bacillus Calmette-Guerin. This integrative recombination event occurs between the phage L5 attP site and the mycobacterial attB site and requires the phage-encoded integrase and mycobacterial-encoded integration host factor mIHF. Here we show that attP, Int-L5, and mIHF assemble into a recombinationally active complex, the intasome, which is capable of attB capture and formation of products. The arm-type integrase binding sites within attP play specialized roles in the formation of specific protein-DNA architectures; the intasome is constructed by the formation of intramolecular integrase bridges between one pair of sites, P4-P5, and the attP core, while an additional pair of sites, P1-P2, is required for interaction with attB.
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Details
Item Type: |
Article
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Status: |
Published |
Creators/Authors: |
Creators | Email | Pitt Username | ORCID |
---|
Peña, CEA | | | | Kahlenberg, JM | | | | Hatfull, GF | gfh@pitt.edu | GFH | |
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Date: |
1 January 1999 |
Date Type: |
Publication |
Journal or Publication Title: |
Journal of Bacteriology |
Volume: |
181 |
Number: |
2 |
Page Range: |
454 - 461 |
DOI or Unique Handle: |
10.1128/jb.181.2.454-461.1999 |
Schools and Programs: |
Dietrich School of Arts and Sciences > Biological Sciences |
Refereed: |
Yes |
ISSN: |
0021-9193 |
MeSH Headings: |
Base Sequence; Binding Sites; Chromosomes, Bacterial--genetics; DNA, Bacterial--genetics; DNA, Bacterial--metabolism; Integrases--metabolism; Lysogeny; Mycobacteriophages--genetics; Mycobacteriophages--metabolism; Mycobacterium bovis--genetics; Mycobacterium bovis--virology; Mycobacterium smegmatis--genetics; Mycobacterium smegmatis--virology; Mycobacterium tuberculosis--genetics; Mycobacterium tuberculosis--virology; Recombination, Genetic; Viral Proteins--genetics; Viral Proteins--metabolism; Virus Integration |
Other ID: |
NLM PMC93398 |
PubMed Central ID: |
PMC93398 |
PubMed ID: |
9882658 |
Date Deposited: |
12 Mar 2013 19:30 |
Last Modified: |
28 Sep 2022 13:21 |
URI: |
http://d-scholarship.pitt.edu/id/eprint/17647 |
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