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Protein-DNA complexes in mycobacteriophage L5 integrative recombination

Peña, CEA and Kahlenberg, JM and Hatfull, GF (1999) Protein-DNA complexes in mycobacteriophage L5 integrative recombination. Journal of Bacteriology, 181 (2). 454 - 461. ISSN 0021-9193

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The temperate mycobacteriophage L5 integrates site specifically into the genomes of Mycobacterium smegmatis, Mycobacterium tuberculosis, and Mycobacterium bovis bacillus Calmette-Guerin. This integrative recombination event occurs between the phage L5 attP site and the mycobacterial attB site and requires the phage-encoded integrase and mycobacterial-encoded integration host factor mIHF. Here we show that attP, Int-L5, and mIHF assemble into a recombinationally active complex, the intasome, which is capable of attB capture and formation of products. The arm-type integrase binding sites within attP play specialized roles in the formation of specific protein-DNA architectures; the intasome is constructed by the formation of intramolecular integrase bridges between one pair of sites, P4-P5, and the attP core, while an additional pair of sites, P1-P2, is required for interaction with attB.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Peña, CEA
Kahlenberg, JM
Hatfull, GFgfh@pitt.eduGFH
Date: 1 January 1999
Date Type: Publication
Journal or Publication Title: Journal of Bacteriology
Volume: 181
Number: 2
Page Range: 454 - 461
DOI or Unique Handle: 10.1128/jb.181.2.454-461.1999
Schools and Programs: Dietrich School of Arts and Sciences > Biological Sciences
Refereed: Yes
ISSN: 0021-9193
MeSH Headings: Base Sequence; Binding Sites; Chromosomes, Bacterial--genetics; DNA, Bacterial--genetics; DNA, Bacterial--metabolism; Integrases--metabolism; Lysogeny; Mycobacteriophages--genetics; Mycobacteriophages--metabolism; Mycobacterium bovis--genetics; Mycobacterium bovis--virology; Mycobacterium smegmatis--genetics; Mycobacterium smegmatis--virology; Mycobacterium tuberculosis--genetics; Mycobacterium tuberculosis--virology; Recombination, Genetic; Viral Proteins--genetics; Viral Proteins--metabolism; Virus Integration
Other ID: NLM PMC93398
PubMed Central ID: PMC93398
PubMed ID: 9882658
Date Deposited: 12 Mar 2013 19:30
Last Modified: 20 Jun 2020 22:55


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