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Rapid film-based determination of antibiotic susceptibilities of Mycobacterium tuberculosis strains by using a luciferase reporter phage and the Bronx Box

Riska, PF and Su, Y and Bardarov, S and Freundlich, L and Sarkis, G and Hatfull, G and Carrière, C and Kumar, V and Chan, J and Jacobs, WR (1999) Rapid film-based determination of antibiotic susceptibilities of Mycobacterium tuberculosis strains by using a luciferase reporter phage and the Bronx Box. Journal of Clinical Microbiology, 37 (4). 1144 - 1149. ISSN 0095-1137

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Detecting antibiotic resistance in Mycobacterium tuberculosis is becoming increasingly important with the global recognition of drug-resistant strains and their adverse impact on clinical outcomes. Current methods of susceptibility testing are either time-consuming or costly; rapid, reliable, simple, and inexpensive methods would be highly desirable, especially in the developing world where most tuberculosis is found. The luciferase reporter phage is a unique reagent well-suited for this purpose: upon infection with viable mycobacteria, it produces quantifiable light which is not observed in mycobacterial cells treated with active antimicrobials. In this report, we describe a modification of our original assay, which allows detection of the emitted light with a Polaroid film box designated the Bronx Box. The technique has been applied to 25 M. tuberculosis reference and clinical strains, and criteria are presented which allow rapid and simple discrimination among strains susceptible or resistant to isoniazid and rifampin, the major antituberculosis agents.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Riska, PF
Su, Y
Bardarov, S
Freundlich, L
Sarkis, G
Hatfull, Ggfh@pitt.eduGFH
Carrière, C
Kumar, V
Chan, J
Jacobs, WR
Date: 1 January 1999
Date Type: Publication
Journal or Publication Title: Journal of Clinical Microbiology
Volume: 37
Number: 4
Page Range: 1144 - 1149
DOI or Unique Handle: 10.1128/jcm.37.4.1144-1149.1999
Schools and Programs: Dietrich School of Arts and Sciences > Biological Sciences
Refereed: Yes
ISSN: 0095-1137
MeSH Headings: Antitubercular Agents--pharmacology; Bacteriophages--enzymology; Bacteriophages--genetics; Drug Resistance, Microbial; Evaluation Studies as Topic; Firefly Luciferin; Genes, Reporter; Humans; Isoniazid--pharmacology; Luciferases--genetics; Luminescent Measurements; Microbial Sensitivity Tests--instrumentation; Microbial Sensitivity Tests--methods; Microbial Sensitivity Tests--statistics & numerical data; Mycobacterium tuberculosis--drug effects; Mycobacterium tuberculosis--genetics; Mycobacterium tuberculosis--isolation & purification; Rifampin--pharmacology; Sensitivity and Specificity; Tuberculosis, Multidrug-Resistant--diagnosis; Tuberculosis, Multidrug-Resistant--microbiology
Other ID: NLM PMC88662
PubMed Central ID: PMC88662
PubMed ID: 10074539
Date Deposited: 12 Mar 2013 19:31
Last Modified: 19 Jul 2020 11:55


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