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L5 luciferase reporter mycobacteriophages: a sensitive tool for the detection and assay of live mycobacteria

Sarkis, GJ and Jacobs, WR and Hatfulll, GF (1995) L5 luciferase reporter mycobacteriophages: a sensitive tool for the detection and assay of live mycobacteria. Molecular Microbiology, 15 (6). 1055 - 1067. ISSN 0950-382X

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Abstract

Recombinant bacteriophages provide efficient delivery systems for introducing reporter genes into specific bacterial hosts. We have constructed mycobacteriophage L5 recombinants carrying the firefly luciferase gene inserted into the tRNA region of the phage genome. Infection of Mycobacterium smegmatis by these phages results in expression of the luciferase gene and light emission. Fortuitously, the luciferase gene is expressed continuously in lysogens surviving infection. Synthesis of luciferase from a mycobacterial promoter created by cloning enables the detection of extremely small numbers of M. smegmatis cells. These reporter phages can be used to discriminate between drug‐sensitive and drug‐resistant strains of M. smegmatis, and may provide tools for the rapid identification and classification of antimycobacterial agents. Copyright © 1995, Wiley Blackwell. All rights reserved

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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Sarkis, GJ
Jacobs, WR
Hatfulll, GF
Date: 1 January 1995
Date Type: Publication
Journal or Publication Title: Molecular Microbiology
Volume: 15
Number: 6
Page Range: 1055 - 1067
DOI or Unique Handle: 10.1111/j.1365-2958.1995.tb02281.x
Schools and Programs: Dietrich School of Arts and Sciences > Biological Sciences
Refereed: Yes
ISSN: 0950-382X
MeSH Headings: Bacterial Typing Techniques; Base Sequence; Drug Resistance, Microbial; Ethambutol--pharmacology; Genes, Insect--genetics; Genes, Reporter--genetics; Genetic Vectors--genetics; Genome, Viral; Isoniazid--pharmacology; Luciferases--biosynthesis; Luciferases--genetics; Luciferases--metabolism; Lysogeny; Molecular Sequence Data; Mycobacteriophages--genetics; Mycobacteriophages--growth & development; Mycobacterium--classification; Mycobacterium--drug effects; Mycobacterium--isolation & purification; Mycobacterium--virology; Promoter Regions, Genetic--genetics; Rifampin--pharmacology; Sensitivity and Specificity; Streptomycin--pharmacology; Transcription, Genetic--drug effects
PubMed ID: 7623662
Date Deposited: 12 Mar 2013 19:33
Last Modified: 02 Feb 2019 15:57
URI: http://d-scholarship.pitt.edu/id/eprint/17654

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