Recombineering mycobacteria and their phages

van Kessel, JC and Marinelli, LJ and Hatfull, GF (2008) Recombineering mycobacteria and their phages. Nature Reviews Microbiology, 6 (11). 851 - 857. ISSN 1740-1526

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Abstract

Bacteriophages are central components in the development of molecular tools for microbial genetics. Mycobacteriophages have proven to be a rich resource for tuberculosis genetics, and the recent development of a mycobacterial recombineering system based on mycobacteriophage Che9c-encoded proteins offers new approaches to mycobacterial mutagenesis. Expression of the phage exonuclease and recombinase substantially enhances recombination frequencies in both fast- and slow-growing mycobacteria, thereby facilitating construction of both gene knockout and point mutants; it also provides a simple and efficient method for constructing mycobacteriophage mutants. Exploitation of host-specific phages thus provides a general strategy for recombineering and mutagenesis in genetically naive systems.

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Details

Item Type:	Article
Status:	Published
Creators/Authors:	Creators Email Pitt Username ORCID van Kessel, JC Marinelli, LJ Hatfull, GF gfh@pitt.edu GFH
Date:	24 October 2008
Date Type:	Publication
Journal or Publication Title:	Nature Reviews Microbiology
Volume:	6
Number:	11
Page Range:	851 - 857
DOI or Unique Handle:	10.1038/nrmicro2014
Schools and Programs:	Dietrich School of Arts and Sciences > Biological Sciences
Refereed:	Yes
ISSN:	1740-1526
Date Deposited:	20 Mar 2013 16:45
Last Modified:	02 Feb 2019 15:58
URI:	http://d-scholarship.pitt.edu/id/eprint/17660

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