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Recombineering mycobacteria and their phages

van Kessel, JC and Marinelli, LJ and Hatfull, GF (2008) Recombineering mycobacteria and their phages. Nature Reviews Microbiology, 6 (11). 851 - 857. ISSN 1740-1526

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Bacteriophages are central components in the development of molecular tools for microbial genetics. Mycobacteriophages have proven to be a rich resource for tuberculosis genetics, and the recent development of a mycobacterial recombineering system based on mycobacteriophage Che9c-encoded proteins offers new approaches to mycobacterial mutagenesis. Expression of the phage exonuclease and recombinase substantially enhances recombination frequencies in both fast- and slow-growing mycobacteria, thereby facilitating construction of both gene knockout and point mutants; it also provides a simple and efficient method for constructing mycobacteriophage mutants. Exploitation of host-specific phages thus provides a general strategy for recombineering and mutagenesis in genetically naive systems.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
van Kessel, JC
Marinelli, LJ
Hatfull, GFgfh@pitt.eduGFH
Date: 24 October 2008
Date Type: Publication
Journal or Publication Title: Nature Reviews Microbiology
Volume: 6
Number: 11
Page Range: 851 - 857
DOI or Unique Handle: 10.1038/nrmicro2014
Schools and Programs: Dietrich School of Arts and Sciences > Biological Sciences
Refereed: Yes
ISSN: 1740-1526
Date Deposited: 20 Mar 2013 16:45
Last Modified: 02 Feb 2019 15:58


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