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Use of in vivo complementation in Mycobacterium tuberculosis to identify a genomic fragment associated with virulence

Pascopella, L and Collins, FM and Martin, JM and Mong Hong Lee, and Hatfull, GF and Stover, CK and Bloom, BR and Jacobs, WR (1994) Use of in vivo complementation in Mycobacterium tuberculosis to identify a genomic fragment associated with virulence. Infection and Immunity, 62 (4). 1313 - 1319. ISSN 0019-9567

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Novel molecular tools and genetic methods were developed to isolate genomic fragments of Mycobacterium tuberculosis that may be associated with virulence. We sought to restore virulence, a characteristic of M. tuberculosis that is correlated with growth rate in mouse spleen and lung tissue, to the avirulent strain H37Ra by complementation. A representative library of the virulent M. tuberculosis strain H37Rv was constructed and transformed into H37Ra. Enrichment for individual faster-growing recombinants was achieved by passage of pools of H37Ra transformants harboring the H37Rv library through mice. A molecular strategy was devised to isolate and clone the H37Rv genomic DNA fragment ivg, which conferred a more rapid in vivo growth rate to H37Ra.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Pascopella, L
Collins, FM
Martin, JM
Mong Hong Lee,
Hatfull, GFgfh@pitt.eduGFH
Stover, CK
Bloom, BR
Jacobs, WR
Date: 1 January 1994
Date Type: Publication
Journal or Publication Title: Infection and Immunity
Volume: 62
Number: 4
Page Range: 1313 - 1319
Schools and Programs: Dietrich School of Arts and Sciences > Biological Sciences
Refereed: Yes
ISSN: 0019-9567
MeSH Headings: Animals; Chromosome Mapping; Gene Library; Genes, Bacterial; Genetic Complementation Test; Mice; Mice, Inbred C57BL; Mycobacterium tuberculosis--genetics; Mycobacterium tuberculosis--growth & development; Mycobacterium tuberculosis--pathogenicity; Recombination, Genetic; Virulence
Other ID: NLM PMC186277
PubMed Central ID: PMC186277
PubMed ID: 8132338
Date Deposited: 19 Mar 2013 18:08
Last Modified: 27 Sep 2022 15:00


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