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A Serratia marcescens PigP Homolog Controls Prodigiosin Biosynthesis, Swarming Motility and Hemolysis and Is Regulated by cAMP-CRP and HexS

Shanks, RMQ and Lahr, RM and Stella, NA and Arena, KE and Brothers, KM and Kwak, DH and Liu, X and Kalivoda, EJ (2013) A Serratia marcescens PigP Homolog Controls Prodigiosin Biosynthesis, Swarming Motility and Hemolysis and Is Regulated by cAMP-CRP and HexS. PLoS ONE, 8 (3).

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Abstract

Swarming motility and hemolysis are virulence-associated determinants for a wide array of pathogenic bacteria. The broad host-range opportunistic pathogen Serratia marcescens produces serratamolide, a small cyclic amino-lipid, that promotes swarming motility and hemolysis. Serratamolide is negatively regulated by the transcription factors HexS and CRP. Positive regulators of serratamolide production are unknown. Similar to serratamolide, the antibiotic pigment, prodigiosin, is regulated by temperature, growth phase, HexS, and CRP. Because of this co-regulation, we tested the hypothesis that a homolog of the PigP transcription factor of the atypical Serratia species ATCC 39006, which positively regulates prodigiosin biosynthesis, is also a positive regulator of serratamolide production in S. marcescens. Mutation of pigP in clinical, environmental, and laboratory strains of S. marcescens conferred pleiotropic phenotypes including the loss of swarming motility, hemolysis, and severely reduced prodigiosin and serratamolide synthesis. Transcriptional analysis and electrophoretic mobility shift assays place PigP in a regulatory pathway with upstream regulators CRP and HexS. The data from this study identifies a positive regulator of serratamolide production, describes novel roles for the PigP transcription factor, shows for the first time that PigP directly regulates the pigment biosynthetic operon, and identifies upstream regulators of pigP. This study suggests that PigP is important for the ability of S. marcescens to compete in the environment. © 2013 Shanks et al.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Shanks, RMQ
Lahr, RMrml40@pitt.eduRML400000-0002-7469-4565
Stella, NAnas92@pitt.eduNAS92
Arena, KE
Brothers, KMkmb227@pitt.eduKMB227
Kwak, DHdhk19@pitt.eduDHK19
Liu, Xxinyuliu@pitt.eduXINYULIU
Kalivoda, EJ
Date: 1 March 2013
Date Type: Publication
Journal or Publication Title: PLoS ONE
Volume: 8
Number: 3
DOI or Unique Handle: 10.1371/journal.pone.0057634
Schools and Programs: Dietrich School of Arts and Sciences > Chemistry
School of Medicine > Ophthalmology
Refereed: Yes
Other ID: NLM PMC3585978
PubMed Central ID: PMC3585978
PubMed ID: 23469212
Date Deposited: 28 Mar 2013 16:47
Last Modified: 04 Feb 2019 15:58
URI: http://d-scholarship.pitt.edu/id/eprint/17872

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