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Human Tendon Stem Cells Better Maintain Their Stemness in Hypoxic Culture Conditions

Zhang, J and Wang, JHC (2013) Human Tendon Stem Cells Better Maintain Their Stemness in Hypoxic Culture Conditions. PLoS ONE, 8 (4).

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Abstract

Tissues and organs in vivo are under a hypoxic condition; that is, the oxygen tension is typically much lower than in ambient air. However, the effects of such a hypoxic condition on tendon stem cells, a recently identified tendon cell, remain incompletely defined. In cell culture experiments, we subjected human tendon stem cells (hTSCs) to a hypoxic condition with 5% O 2 , while subjecting control cells to a normaxic condition with 20% O 2 . We found that hTSCs at 5% O 2 had significantly greater cell proliferation than those at 20% O 2 . Moreover, the expression of two stem cell marker genes, Nanog and Oct-4, was upregulated in the cells cultured in 5% O 2 . Finally, in cultures under 5% O 2 , more hTSCs expressed the stem cell markers nucleostemin, Oct-4, Nanog and SSEA-4. In an in vivo experiment, we found that when both cell groups were implanted with tendon-derived matrix, more tendon-like structures formed in the 5% O 2 treated hTSCs than in 20% O 2 treated hTSCs. Additionally, when both cell groups were implanted with Matrigel, the 5% O 2 treated hTSCs showed more extensive formation of fatty, cartilage-like and bone-like tissues than the 20% O 2 treated cells. Together, the findings of this study show that oxygen tension is a niche factor that regulates the stemness of hTSCs, and that less oxygen is better for maintaining hTSCs in culture and expanding them for cell therapy of tendon injuries. © 2013 Wang, Zhang.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Zhang, Jjianying@pitt.eduJIANYING
Wang, JHCwanghc@pitt.eduWANGHC
Contributors:
ContributionContributors NameEmailPitt UsernameORCID
EditorAgarwal, SudhaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Date: 16 April 2013
Date Type: Publication
Journal or Publication Title: PLoS ONE
Volume: 8
Number: 4
DOI or Unique Handle: 10.1371/journal.pone.0061424
Schools and Programs: School of Medicine > Orthopaedic Surgery
School of Medicine > Physical Medicine and Rehabilitation
School of Medicine > Surgery
Swanson School of Engineering > Bioengineering
Swanson School of Engineering > Materials Science and Engineering
Swanson School of Engineering > Mechanical Engineering
Refereed: Yes
Date Deposited: 03 May 2013 20:57
Last Modified: 16 Mar 2019 15:55
URI: http://d-scholarship.pitt.edu/id/eprint/18444

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