Sun, Tao
(2013)
FUNCTIONS AND REGULATION OF CD8 T CELLS DURING MURINE INFECTION WITH MYCOBACTERIUM TUBERCULOSIS.
Master's Thesis, University of Pittsburgh.
(Unpublished)
Abstract
Mycobacterium tuberculosis (Mtb), causing tuberculosis (TB), is estimated to infect one third of the human population, and kills about 1.8 million people per year. CD8 T cells are an essential component of adaptive immunity against Mtb. Mtb-specific CD8 T cells in both mice and non-human primates have been found to exhibit two distinct functional phenotypes: either cytokine secreting or cytolytic activity. In this study, we established an in vitro culture system to expand and differentiate cells and then examined the effects of specific cytokines on priming of CD8 T cells in both vitro and vivo. We hypothesize that these cytokines affect the priming of Mtb-specific CD8 T cell responses. In addition, we optimized the immunohistochemical staining for CD8 on granulomas from infected non-human primates, which will enable us to better characterize the distribution and functions of CD8 T cells within granulomas. Finally, we determined whether IL-21 plays a role in bacterial control and immune responses in mice infected by Mtb. In summary, our study will contribute to a better understanding in the roles of CD8 T cells against Mtb and mechanisms by which they are modulated during the infection, which may aid the development of protective therapies and vaccines.
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Details
| Item Type: |
University of Pittsburgh ETD
|
| Status: |
Unpublished |
| Creators/Authors: |
|
| ETD Committee: |
|
| Date: |
2013 |
| Date Type: |
Publication |
| Defense Date: |
22 April 2013 |
| Approval Date: |
3 May 2013 |
| Submission Date: |
2 May 2013 |
| Access Restriction: |
5 year -- Restrict access to University of Pittsburgh for a period of 5 years. |
| Number of Pages: |
61 |
| Institution: |
University of Pittsburgh |
| Schools and Programs: |
School of Medicine > Immunology |
| Degree: |
MS - Master of Science |
| Thesis Type: |
Master's Thesis |
| Refereed: |
Yes |
| Uncontrolled Keywords: |
Tuberculosis, CD8 T cells |
| Date Deposited: |
03 May 2013 14:40 |
| Last Modified: |
03 May 2018 05:15 |
| URI: |
http://d-scholarship.pitt.edu/id/eprint/18664 |
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