Kellner, Sean
(2013)
Autoregulation of c-Fes by intramolecular interaction of the F-BAR and SH2 domains: a novel role for the unique N-terminal region.
Master's Thesis, University of Pittsburgh.
(Unpublished)
Abstract
The c-Fes protein is a structurally unique non-receptor protein-tyrosine kinase encoded by the fes/fps proto-oncogene and is expressed in myeloid hematopoietic, vascular endothelial, and some epithelial and neuronal cell types. Downstream of a number of cytokines and growth factors, c-Fes plays a role in regulation of cell growth, differentiation, and chemotaxis. Dysregulation of c-Fes activity is implicated in a number of cancers, including acute myelogenous leukemia (AML) and some renal carcinomas. Structurally, c-Fes includes an amino-terminal F-BAR region—consisting of a Fes/CIP4 homology (FCH) domain and the first of two coiled-coil motifs (CC1)—followed by CC2 and the carboxy-terminal Src homology 2 (SH2) domain closely linked to a typical bilobate kinase domain (KD).
Unlike its oncogenic retroviral orthologs (v-Fes/Fps), c-Fes kinase activity is tightly regulated in vivo, yet lacks known negative regulatory features common to other cytoplasmic tyrosine kinases, such as a SH3 domain or regulatory tail tyrosine. In fact, a c-Fes truncation containing only the SH2 and KD is constitutively active which suggests a possible auto-inhibitory role for the unique N-terminal region. Deletion and mutation experiments reveal that loss of the predicted CC1 structure leads to upregulation similar to the c-Fes SH2-KD truncation. This would be the first case of autoinhibition by the coiled-coil; however, it is still unclear how the F-BAR acts in cis to accomplish autoinhibition. Substitution of the native c-Fes SH2 with that of v-Src (but not homologous v-Fps) displays an uninhibited catalytic activity level analogous to the CC1 mutant, suggesting interdomain interaction between the F-BAR and SH2/KD may contribute to an inactive conformation. I collected evidence for this mechanism and began to develop tools to define the putative F-BAR:SH2 interface utilizing the bimolecular fluorescence complementation (BiFC) technique. Demonstrated by BiFC in 293T cells, the F-BAR interacts with the SH2 domain in a CC1-dependent manner. It may be that CC1 forms a critical interface with αB of the SH2 as preliminary data suggest that v-Src-mimetic mutations in αB weaken CC1-SH2 recognition. Further characterization of the specific intramolecular interactions involved in c-Fes regulation would advance new paradigms in modular domain relationships and could expand cancer drug discovery efforts.
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Details
| Item Type: |
University of Pittsburgh ETD
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| Status: |
Unpublished |
| Creators/Authors: |
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| ETD Committee: |
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| Date: |
6 May 2013 |
| Date Type: |
Publication |
| Defense Date: |
2 May 2013 |
| Approval Date: |
6 May 2013 |
| Submission Date: |
6 May 2013 |
| Access Restriction: |
No restriction; Release the ETD for access worldwide immediately. |
| Number of Pages: |
77 |
| Institution: |
University of Pittsburgh |
| Schools and Programs: |
School of Medicine > Integrative Molecular Biology |
| Degree: |
MS - Master of Science |
| Thesis Type: |
Master's Thesis |
| Refereed: |
Yes |
| Uncontrolled Keywords: |
fes, tyrosine kinase, f-bar, sh2, autoregulation, auto-regulation, coiled-coil, acute myelogenous leukemia, colorectal cancer, bimolecular fluorescence complementation, bifc |
| Date Deposited: |
06 May 2013 19:54 |
| Last Modified: |
15 Nov 2016 14:12 |
| URI: |
http://d-scholarship.pitt.edu/id/eprint/18670 |
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