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Genetically encoded redox sensor identifies the role of ROS in degenerative and mitochondrial disease pathogenesis

Liu, Z and Celotto, AM and Romero, G and Wipf, P and Palladino, MJ (2012) Genetically encoded redox sensor identifies the role of ROS in degenerative and mitochondrial disease pathogenesis. Neurobiology of Disease, 45 (1). 362 - 368. ISSN 0969-9961

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Abstract

Mitochondrial dysfunction plays an important role in the pathogenesis of neurodegenerative diseases, numerous other disease states and senescence. The ability to monitor reactive oxygen species (ROS) within tissues and over time in animal model systems is of significant research value. Recently, redox-sensitive fluorescent proteins have been developed. Transgenic flies expressing genetically encoded redox-sensitive GFPs (roGFPs) targeted to the mitochondria function as a useful in vivo assay of mitochondrial dysfunction and ROS. We have generated transgenic flies expressing a mitochondrial-targeted roGFP2, demonstrated its responsiveness to redox changes in cultured cells and in vivo and utilized this protein to discover elevated ROS as a contributor to pathogenesis in a characterized neurodegeneration mutant and in a model of mitochondrial encephalomyopathy. These studies identify the role of ROS in pathogenesis associated with mitochondrial disease and demonstrate the utility of genetically encoded redox sensors in Drosophila. © 2011 Elsevier Inc.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Liu, Z
Celotto, AM
Romero, G
Wipf, Ppwipf@pitt.eduPWIPF
Palladino, MJ
Date: 1 January 2012
Date Type: Publication
Journal or Publication Title: Neurobiology of Disease
Volume: 45
Number: 1
Page Range: 362 - 368
DOI or Unique Handle: 10.1016/j.nbd.2011.08.022
Schools and Programs: Dietrich School of Arts and Sciences > Chemistry
Refereed: Yes
ISSN: 0969-9961
MeSH Headings: Animals; Drosophila melanogaster; Mitochondria--genetics; Mitochondria--metabolism; Mitochondrial Diseases--genetics; Mitochondrial Diseases--metabolism; Neurodegenerative Diseases--genetics; Neurodegenerative Diseases--metabolism; Neurons--metabolism; Oxidation-Reduction; Reactive Oxygen Species--metabolism
Other ID: NLM NIHMS321481, NLM PMC3225579
PubMed Central ID: PMC3225579
PubMed ID: 21889980
Date Deposited: 28 May 2013 14:57
Last Modified: 02 Feb 2019 16:56
URI: http://d-scholarship.pitt.edu/id/eprint/18734

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