Controlled Delivery of Sonic Hedgehog Morphogen and Its Potential for Cardiac Repair

Johnson, NR and Wang, Y (2013) Controlled Delivery of Sonic Hedgehog Morphogen and Its Potential for Cardiac Repair. PLoS ONE, 8 (5).

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Abstract

The morphogen Sonic hedgehog (Shh) holds great promise for repair or regeneration of tissues suffering ischemic injury, however clinical translation is limited by its short half-life in the body. Here, we describe a coacervate delivery system which incorporates Shh, protects it from degradation, and sustains its release for at least 3 weeks. Shh released from the coacervate stimulates cardiac fibroblasts to upregulate the expression of multiple trophic factors including VEGF, SDF-1α, IGF-1, and Shh itself, for at least 48 hours. Shh coacervate also demonstrates cytoprotective effects for cardiomyocytes in a hydrogen peroxide-induced oxidative stress environment. In each of these studies the bioactivity of the Shh coacervate is enhanced compared to free Shh. These results warrant further investigation of the in vivo efficacy of Shh coacervate for cardiac repair. © 2013 Johnson, Wang.

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Details

Item Type:	Article
Status:	Published
Creators/Authors:	Creators Email Pitt Username ORCID Johnson, NR Wang, Y yaw20@pitt.edu YAW20 orcid.org/0000-0003-2067-382X
Contributors:	Contribution Contributors Name Email Pitt Username ORCID Editor Qin, Gangjian UNSPECIFIED UNSPECIFIED UNSPECIFIED
Centers:	Other Centers, Institutes, Offices, or Units > McGowan Institute for Regenerative Medicine
Date:	14 May 2013
Date Type:	Publication
Journal or Publication Title:	PLoS ONE
Volume:	8
Number:	5
DOI or Unique Handle:	10.1371/journal.pone.0063075
Schools and Programs:	School of Medicine > Surgery Swanson School of Engineering > Bioengineering Swanson School of Engineering > Chemical Engineering Swanson School of Engineering > Petroleum Engineering
Refereed:	Yes
Date Deposited:	12 Jun 2013 20:49
Last Modified:	13 Oct 2017 22:57
URI:	http://d-scholarship.pitt.edu/id/eprint/18834

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