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Aerosolized Influenza Infection During Late Gestation Pregnancy in the Ferrot Model

Gage, Emily (2013) Aerosolized Influenza Infection During Late Gestation Pregnancy in the Ferrot Model. Master's Thesis, University of Pittsburgh. (Unpublished)

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Abstract

Human pregnancy is known to predispose women to disproportionate morbidity and mortality from influenza infection. Limited investigation has been performed to understand the mechanism(s) of increased risk. Investigation of a pregnant ferret model was undertaken using aerosolized 2009 pH1N1 influenza. Pregnant ferrets demonstrated worse clinical disease and increased pro-inflammatory cytokine gene expression levels, notably IL-6 and IL-10 in the lung and at the maternal/fetal interface but not the spleen. Influenza virus was detected by semi-quantitative RT-PCR in many tissues including the placenta and kits but not the uterus of the non-pregnant ferrets, suggesting placental-fetal transmission. No obvious difference in lung pathology was observed between the pregnant and age-matched controls. Further model development is warranted to elucidate mechanisms that cause greater disease during pregnancy and effects of maternal disease on fetal outcomes. Ultimately, this model would further aid in investigating countermeasures against influenza during pregnancy. The public health significance of this study is that by establishing a pertinent influenza pregnant animal model, answers to translational questions about the effectiveness of therapeutics and when treatment would be most effective could be investigated, potentially lowering the amount of lives lost,hospitalization rates, and economic burden caused by this health disparity.


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Details

Item Type: University of Pittsburgh ETD
Status: Unpublished
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Gage, Emilygage.emily@Gmail.com
ETD Committee:
TitleMemberEmail AddressPitt UsernameORCID
Committee ChairCole, Kellystefcole@cvr.pitt.eduSTEFCOLE
Committee MemberHartman, Amyhartman2@pitt.eduHARTMAN2
Committee MemberKolls, Jayjay.kolls@chp.eduJKK23
Committee MemberReinhart, Todd A.reinhar@pitt.eduREINHAR
Date: 27 June 2013
Date Type: Publication
Defense Date: 3 April 2013
Approval Date: 27 June 2013
Submission Date: 10 June 2013
Access Restriction: 1 year -- Restrict access to University of Pittsburgh for a period of 1 year.
Number of Pages: 77
Institution: University of Pittsburgh
Schools and Programs: Graduate School of Public Health > Infectious Diseases and Microbiology
Degree: MS - Master of Science
Thesis Type: Master's Thesis
Refereed: Yes
Uncontrolled Keywords: pregnancy, ferret model , influenza
Date Deposited: 27 Jun 2013 18:01
Last Modified: 19 Dec 2016 14:40
URI: http://d-scholarship.pitt.edu/id/eprint/18940

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