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In Vivo Local Drug Delivery of Non-electroactive Species

Boyer, Nichole E. (2013) In Vivo Local Drug Delivery of Non-electroactive Species. Master's Thesis, University of Pittsburgh. (Unpublished)

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Abstract

Pressure ejection is the controlled expulsion of substances using applied air pressure from pulled multibarrel glass capillaries. The technique is frequently employed in neuroscience for the localized delivery of reagents to select brain regions of interest. Our lab has recently adapted a method to prevent unintentional leaking of reagent into the recording site. Here, those principles have been adapted to allow the local delivery of non-electroactive substances in vivo. A pressure-ejected system was coupled to fast scan cyclic voltammetry for real-time monitoring of ejections and a dye was introduced into the pipet barrels for visualization of ejection progress. This study shows that the dye had no effect on the electrically evoked dopamine response. Additionally, it shows the method proposed is applicable for fabrication and local employment of electroinactive reagents delivery of in vivo.

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Details

Item Type: University of Pittsburgh ETD
Status: Unpublished
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Boyer, Nichole E.neb36@pitt.eduNEB36
ETD Committee:
TitleMemberEmail AddressPitt UsernameORCID
Committee ChairMichael, Adrian C.amichael@pitt.eduAMICHAEL
Committee MemberAmemiya, Shigeruamemiya@pitt.eduAMEMIYA
Committee MemberWeber, Stevesweber@pitt.eduSWEBER
Date: 22 September 2013
Date Type: Publication
Defense Date: 29 July 2013
Approval Date: 22 September 2013
Submission Date: 30 July 2013
Access Restriction: No restriction; Release the ETD for access worldwide immediately.
Number of Pages: 49
Institution: University of Pittsburgh
Schools and Programs: Dietrich School of Arts and Sciences > Chemistry
Degree: MS - Master of Science
Thesis Type: Master's Thesis
Refereed: Yes
Uncontrolled Keywords: Pressure ejection, raclopride, dopamine
Date Deposited: 22 Sep 2013 21:13
Last Modified: 15 Nov 2016 14:14
URI: http://d-scholarship.pitt.edu/id/eprint/19521

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