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The Expression and Localization of N-Myc Downstream-Regulated Gene 1 in Human Trophoblasts

Shi, XH and Larkin, JC and Chen, B and Sadovsky, Y (2013) The Expression and Localization of N-Myc Downstream-Regulated Gene 1 in Human Trophoblasts. PLoS ONE, 8 (9).

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Abstract

The protein N-Myc downstream-regulated gene 1 (NDRG1) is implicated in the regulation of cell proliferation, differentiation, and cellular stress response. NDRG1 is expressed in primary human trophoblasts, where it promotes cell viability and resistance to hypoxic injury. The mechanism of action of NDRG1 remains unknown. To gain further insight into the intracellular action of NDRG1, we analyzed the expression pattern and cellular localization of endogenous NDRG1 and transfected Myc-tagged NDRG1 in human trophoblasts exposed to diverse injuries. In standard conditions, NDRG1 was diffusely expressed in the cytoplasm at a low level. Hypoxia or the hypoxia mimetic cobalt chloride, but not serum deprivation, ultraviolet (UV) light, or ionizing radiation, induced the expression of NDRG1 in human trophoblasts and the redistribution of NDRG1 into the nucleus and cytoplasmic membranes associated with the endoplasmic reticulum (ER) and microtubules. Mutation of the phosphopantetheine attachment site (PPAS) within NDRG1 abrogated this pattern of redistribution. Our results shed new light on the impact of cell injury on NDRG1 expression patterns, and suggest that the PPAS domain plays a key role in NDRG1's subcellular distribution. © 2013 Shi et al.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Shi, XH
Larkin, JCjcl56@pitt.eduJCL56
Chen, B
Sadovsky, Yyos14@pitt.eduYOS14
Date: 16 September 2013
Date Type: Publication
Journal or Publication Title: PLoS ONE
Volume: 8
Number: 9
DOI or Unique Handle: 10.1371/journal.pone.0075473
Schools and Programs: School of Medicine > Microbiology and Molecular Genetics
School of Medicine > Obstetrics, Gynecology, and Reproductive Sciences
Refereed: Yes
Date Deposited: 10 Oct 2013 14:32
Last Modified: 13 Oct 2017 20:56
URI: http://d-scholarship.pitt.edu/id/eprint/19824

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