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HPLC determinations of enantiomeric ratios

Wipf, P and Werner, S and Twining, LA and Kendall, C (2007) HPLC determinations of enantiomeric ratios. Chirality, 19 (1). 5 - 9. ISSN 0899-0042

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Evaporative light-scattering detection (ELSD) is widely applied in the HPLC analysis of organic compounds lacking a UY chromophore. However, this detection method is generally unsuitable for determination of enantiomeric ratios (er). The er calculated from a UV trace and an ELS trace of the same compound differs significantly. Because of the nonlinear concentration response of the ELS detector, a compound with an er of 95:5 appears to be enantiomerically pure by ELS detection. It is possible to obtain a calibration curve and to calculate a correction factor, but this procedure is time consuming and therefore not very practical for routine analyses. In contrast, a charged aerosol detector allows a very accurate determination of the enantiomeric ratios. Like the ELS detection, the CA detection is independent of the chromophore properties of the substrate. Therefore, we recommend the use of CA instead of ELS detection for determination of the enantiomeric ratios of non-UV active compounds. © 2006 Wiley-Liss, Inc.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Wipf, Ppwipf@pitt.eduPWIPF
Werner, S
Twining, LA
Kendall, C
Date: 1 January 2007
Date Type: Publication
Journal or Publication Title: Chirality
Volume: 19
Number: 1
Page Range: 5 - 9
DOI or Unique Handle: 10.1002/chir.20331
Schools and Programs: Dietrich School of Arts and Sciences > Chemistry
Refereed: Yes
ISSN: 0899-0042
MeSH Headings: Chromatography, High Pressure Liquid--methods; Light; Scattering, Radiation; Stereoisomerism
PubMed ID: 17089337
Date Deposited: 28 Oct 2013 22:26
Last Modified: 07 Dec 2019 15:55


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