Link to the University of Pittsburgh Homepage
Link to the University Library System Homepage Link to the Contact Us Form

Elucidating the Role of the Humoral Response in Mycobacterium tuberculosis infected Cynomolgus Macaques

Phuah, Jiayao (2013) Elucidating the Role of the Humoral Response in Mycobacterium tuberculosis infected Cynomolgus Macaques. Doctoral Dissertation, University of Pittsburgh. (Unpublished)

Primary Text

Download (4MB) | Preview


Mycobacterium tuberculosis remains as a global burden today with an estimated one-third of the global population being infected and at risk of developing active infection. Most studies in the immunology and pathogenesis of tuberculosis have revealed the importance of cellular immunity in controlling the infection. However, the role of the humoral immune response is poorly understood, particularly in the primate models of M. tuberculosis infection. The primary goal of this thesis was to understand how B cells and antibody contribute towards containment of M. tuberculosis within the cynomolgus macaque model of infection. The thesis starts off by characterizing B cells and antibody profiles within the granulomas of M. tuberculosis infected cynomolgus macaques. B cells were noted organize themselves into clusters that resemble germinal centers found in lymphoid organs or in chronic autoimmune conditions, within the granuloma. The effect of B cell depletion on the outcome of M. tuberculosis infection in cynomolgus macaques was also performed. The study findings suggest that B cells and antibody contribute very little in terms of disease control in the early stages of natural M. tuberculosis infection. However, subtle differences such as a slight increase in bacterial burden within individual granulomas and altered cytokine correlations within individual granulomas were noted. Macrophage behavior in the absence of B cells and antibody was also studied using
granuloma tissue derived from the B cell depletion study. No differences were found within the macrophages in the absence of B cells at least at the acute stage of infection. The experiments detailed in this thesis suggest that the humoral response is not crucial towards M. tuberculosis control in the early stages of natural infection. However, the findings in this thesis suggest that B cells and antibody may play a role in long term chronic control of tuberculosis infection.


Social Networking:
Share |


Item Type: University of Pittsburgh ETD
Status: Unpublished
CreatorsEmailPitt UsernameORCID
ETD Committee:
TitleMemberEmail AddressPitt UsernameORCID
Committee ChairFlynn, JoAnne Ljoanne@pitt.eduJOANNE
Committee MemberCarney,
Committee MemberMorel, Penelope Amorel@pitt.eduMOREL
Committee MemberKhader, Shabaana
Committee MemberKane, Lawrencelkane@pitt.eduLKANE
Committee MemberNorris, Karen Akan1@pitt.eduKAN1
Date: 18 October 2013
Date Type: Publication
Defense Date: 30 September 2013
Approval Date: 18 October 2013
Submission Date: 17 October 2013
Access Restriction: 2 year -- Restrict access to University of Pittsburgh for a period of 2 years.
Number of Pages: 182
Institution: University of Pittsburgh
Schools and Programs: School of Medicine > Immunology
Degree: PhD - Doctor of Philosophy
Thesis Type: Doctoral Dissertation
Refereed: Yes
Uncontrolled Keywords: B cells; Tuberculosis; Non human primate
Date Deposited: 18 Oct 2013 13:06
Last Modified: 15 Nov 2016 14:15


Monthly Views for the past 3 years

Plum Analytics

Actions (login required)

View Item View Item