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A novel nonhuman primate model for influenza transmission

Moncla, LH and Ross, TM and Dinis, JM and Weinfurter, JT and Mortimer, TD and Schultz-Darken, N and Brunner, K and Capuano, SV and Boettcher, C and Post, J and Johnson, M and Bloom, CE and Weiler, AM and Friedrich, TC (2013) A novel nonhuman primate model for influenza transmission. PLoS ONE, 8 (11).

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Abstract

Studies of influenza transmission are necessary to predict the pandemic potential of emerging influenza viruses. Currently, both ferrets and guinea pigs are used in such studies, but these species are distantly related to humans. Nonhuman primates (NHP) share a close phylogenetic relationship with humans and may provide an enhanced means to model the virological and immunological events in influenza virus transmission. Here, for the first time, it was demonstrated that a human influenza virus isolate can productively infect and be transmitted between common marmosets (Callithrix jacchus), a New World monkey species. We inoculated four marmosets with the 2009 pandemic virus A/California/07/2009 (H1N1pdm) and housed each together with a naïve cage mate. We collected bronchoalveolar lavage and nasal wash samples from all animals at regular intervals for three weeks post-inoculation to track virus replication and sequence evolution. The unadapted 2009 H1N1pdm virus replicated to high titers in all four index animals by 1 day post-infection. Infected animals seroconverted and presented human-like symptoms including sneezing, nasal discharge, labored breathing, and lung damage. Transmission occurred in one cohabitating pair. Deep sequencing detected relatively few genetic changes in H1N1pdm viruses replicating in any infected animal. Together our data suggest that human H1N1pdm viruses require little adaptation to replicate and cause disease in marmosets, and that these viruses can be transmitted between animals. Marmosets may therefore be a viable model for studying influenza virus transmission. © 2013 Moncla et al.

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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Moncla, LH
Ross, TM
Dinis, JM
Weinfurter, JT
Mortimer, TD
Schultz-Darken, N
Brunner, K
Capuano, SV
Boettcher, C
Post, J
Johnson, M
Bloom, CE
Weiler, AM
Friedrich, TC
Contributors:
ContributionContributors NameEmailPitt UsernameORCID
EditorPekosz, AndrewUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Date: 14 November 2013
Date Type: Publication
Journal or Publication Title: PLoS ONE
Volume: 8
Number: 11
DOI or Unique Handle: 10.1371/journal.pone.0078750
Schools and Programs: School of Medicine > Microbiology and Molecular Genetics
Refereed: Yes
Date Deposited: 30 Jan 2014 17:39
Last Modified: 02 Feb 2019 16:57
URI: http://d-scholarship.pitt.edu/id/eprint/20395

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