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Muscle-derived stem/progenitor cell dysfunction in Zmpste24-deficient progeroid mice limits muscle regeneration

Song, M and Lavasani, M and Thompson, SD and Lu, A and Ahani, B and Huard, J (2013) Muscle-derived stem/progenitor cell dysfunction in Zmpste24-deficient progeroid mice limits muscle regeneration. Stem Cell Research and Therapy, 4 (2).

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Abstract

Introduction. Loss of adult stem cell function during aging contributes to impaired tissue regeneration. Here, we tested the aging-related decline in regeneration potential of adult stem cells residing in the skeletal muscle. Methods. We isolated muscle-derived stem/progenitor cells (MDSPCs) from progeroid Zmpste24-deficient mice (Zmpste24§ssup§-/-§esup§) with accelerated aging phenotypes to investigate whether mutation in lamin A has an adverse effect on muscle stem/progenitor cell function. Results: Our results indicate that MDSPCs isolated from Zmpste24§ssup§-/- §esup§mice show reduced proliferation and myogenic differentiation. In addition, Zmpste24§ssup§-/- §esup§MDSPCs showed impaired muscle regeneration, with a limited engraftment potential when transplanted into dystrophic muscle, compared with wild-type (WT) MDSPCs. Exposure of progeroid Zmpste24§ssup§-/- §esup§MDSPCs to WT MDSPCs rescued the myogenic differentiation defect in vitro. Conclusions: These results demonstrate that adult stem/progenitor cell dysfunction contributes to impairment of tissue regeneration and suggest that factors secreted by functional cells are indeed important for the therapeutic effect of adult stem cells. © 2013 Song et al.; licensee BioMed Central Ltd.


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Details

Item Type: Article
Status: Published
Creators/Authors:
CreatorsEmailPitt UsernameORCID
Song, M
Lavasani, M
Thompson, SD
Lu, A
Ahani, B
Huard, J
Centers: Other Centers, Institutes, or Units > Stem Cell Research Center
Date: 8 May 2013
Date Type: Publication
Access Restriction: No restriction; Release the ETD for access worldwide immediately.
Journal or Publication Title: Stem Cell Research and Therapy
Volume: 4
Number: 2
DOI or Unique Handle: 10.1186/scrt183
Institution: University of Pittsburgh
Schools and Programs: School of Medicine > Microbiology and Molecular Genetics
School of Medicine > Orthopaedic Surgery
Swanson School of Engineering > Bioengineering
Refereed: Yes
PubMed ID: 23531345
Date Deposited: 31 Mar 2014 15:15
Last Modified: 20 Dec 2018 00:55
URI: http://d-scholarship.pitt.edu/id/eprint/20800

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