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Tendon graft revitalization using adult anterior cruciate ligament (ACL)-derived CD34+ cell sheets for ACL reconstruction

Mifune, Y and Matsumoto, T and Takayama, K and Terada, S and Sekiya, N and Kuroda, R and Kurosaka, M and Fu, FH and Huard, J (2013) Tendon graft revitalization using adult anterior cruciate ligament (ACL)-derived CD34+ cell sheets for ACL reconstruction. Biomaterials, 34 (22). 5476 - 5487. ISSN 0142-9612

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As a new strategy for enhancing recovery after anterior cruciate ligament (ACL) reconstruction, we developed a cell sheet wrapped graft using ACL-derived CD34+ cells. Our results from an exvivo cell migration assay demonstrated gradual cell migration into the graft from the cell sheet, and an enzyme-linked immunosorbent assay indicated that the cell sheet cultures secreted a significantly greater amount of VEGF than typical monolayer cultures. In the invivo study, the rats received one of three possible ACL reconstruction treatments; 1) a tendon graft wrapped in an ACL-derived CD34+ cell sheet, 2) a tendon graft and ACL-derived CD34+ cell injection, or 3) the control group that received just the tendon graft. The invivo studies revealed that the ACL-derived CD34+ cell sheet wrapped grafts demonstrated a greater number of the cells derived from the cell sheets incorporated within the bone tunnel site and grafted tendon. We believe the incorporated CD34+ cells enhanced the healing of both the bone-tendon junction and the grafted tendon in the reconstructed rat ACLs by increasing proprioceptive recovery, graft maturation, and biomechanical strength. ACL-derived CD34+ cell sheet wrapped grafts could become a promising strategy to revitalize tendon autografts and recovery after ACL reconstruction resulting in superior and quicker recovery in patients requiring ACL reconstruction. © 2013 Elsevier Ltd.


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Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Mifune, Y
Matsumoto, T
Takayama, K
Terada, S
Sekiya, N
Kuroda, R
Kurosaka, M
Fu, FHffu@pitt.eduFFU
Huard, J
Centers: Other Centers, Institutes, Offices, or Units > Stem Cell Research Center
Date: 1 July 2013
Date Type: Publication
Journal or Publication Title: Biomaterials
Volume: 34
Number: 22
Page Range: 5476 - 5487
DOI or Unique Handle: 10.1016/j.biomaterials.2013.04.013
Schools and Programs: School of Medicine > Biochemistry and Molecular Genetics
School of Medicine > Orthopaedic Surgery
Swanson School of Engineering > Bioengineering
Refereed: Yes
ISSN: 0142-9612
PubMed ID: 23632324
Date Deposited: 31 Mar 2014 15:01
Last Modified: 03 Feb 2019 10:55


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