Link to the University of Pittsburgh Homepage
Link to the University Library System Homepage Link to the Contact Us Form

Isolation of muscle-derived stem/progenitor cells based on adhesion characteristics to collagen-coated surfaces

Lavasani, M and Lu, A and Thompson, SD and Robbins, PD and Huard, J and Niedernhofer, LJ (2013) Isolation of muscle-derived stem/progenitor cells based on adhesion characteristics to collagen-coated surfaces. Methods in Molecular Biology, 976. 53 - 65. ISSN 1064-3745

[img] Plain Text (licence)
Available under License : See the attached license file.

Download (1kB)


Our lab developed and optimized a method, known as the modified pre-plate technique, to isolate stem/ progenitor cells from skeletal muscle. This method separates different populations of myogenic cells based on their propensity to adhere to a collagen I-coated surface. Based on their surface markers and stem-like properties, including self-renewal, multi-lineage differentiation, and ability to promote tissue regeneration, the last cell fraction or slowest to adhere to the collagen-coated surface (pre-plate 6; pp6) appears to be early, quiescent progenitor cells termed muscle-derived stem/progenitor cells (MDSPCs). The cell fractions preceding pp6 (pp1-5) are likely populations of more committed (differentiated) cells, includingfibroblast- and myoblast-like cells. This technique may be used to isolate MDSPCs from skeletal muscle of humans or mice regardless of age, sex or disease state, although the yield of MDSPCs varies with age and health. MDSPCs can be used for regeneration of a variety of tissues including bone, articular cartilage, skeletal and cardiac muscle, and nerve. MDSPCs are currently being tested in clinical trials for treatment of urinary incontinence and myocardial infarction. MDSPCs from young mice have also been demonstrated to extend life span and healthspan in mouse models of accelerated aging through an apparent paracrine/ endocrine mechanism. Here we detail methods for isolation and characterization of MDSPCs. © Springer Science+Business Media, LLC 2013.


Social Networking:
Share |


Item Type: Article
Status: Published
CreatorsEmailPitt UsernameORCID
Lavasani, M
Lu, A
Thompson, SD
Robbins, PDprobb@pitt.eduPROBB
Huard, J
Niedernhofer, LJniedernh@pitt.eduNIEDERNH
ContributionContributors NameEmailPitt UsernameORCID
Centers: Other Centers, Institutes, Offices, or Units > Hillman Cancer Center
Other Centers, Institutes, Offices, or Units > Stem Cell Research Center
Date: 1 January 2013
Date Type: Publication
Journal or Publication Title: Methods in Molecular Biology
Volume: 976
Page Range: 53 - 65
DOI or Unique Handle: 10.1007/978-1-62703-317-6_5
Schools and Programs: School of Medicine > Microbiology and Molecular Genetics
School of Medicine > Orthopaedic Surgery
Refereed: Yes
ISSN: 1064-3745
MeSH Headings: Animals; Cell Adhesion--physiology; Cell Differentiation; Cell Separation--methods; Cells, Cultured; Collagen--metabolism; Mice; Muscle, Skeletal--cytology; Muscle, Skeletal--physiology; Stem Cells--cytology; Stem Cells--physiology
Other ID: NLM NIHMS491012, NLM PMC3731996
PubMed Central ID: PMC3731996
PubMed ID: 23400434
Date Deposited: 31 Mar 2014 15:20
Last Modified: 03 Feb 2019 06:55


Monthly Views for the past 3 years

Plum Analytics

Actions (login required)

View Item View Item